Validating Bioluminescence Imaging as a High-Throughput, Quantitative Modality for Assessing Tumor Burden
Validating Bioluminescence Imaging as a High-Throughput, Quantitative Modality for Assessing Tumor Burden
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DOI：
10.1162/1535350041464865
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发表时间：
2004-04-01
影响因子：
2.8
通讯作者：
Mason, Ralph P.
中科院分区：
文献类型：
--
作者：
Paroo, Zain;Bollinger, Robert A.;Mason, Ralph P.
生物发光成像（BLI）是一种高度敏感的工具，用于可视化肿瘤，肿瘤发展，转移扩散和对治疗的反应。尽管BLI因其明显的简单性和易于实施而引起了很大的兴奋，但很少有严格的研究来验证测量结果。在这里，我们的特点的性质，生物发光输出的小鼠皮下转移酶表达肿瘤超过4周的时间。腹膜内或直接瘤内给药后，有一个高度动态的光发射动力学曲线。尽管生物发光具有可变性，但测定了卡尺测量的肿瘤体积与峰值光信号、光信号曲线下面积和特定时间点的光发射之间的强相关性（r > 0.8，p <0.001）。此外，用生物发光监测的肿瘤生长概况与卡尺测量非常相似。该研究表明，尽管生物发光的动态和可变性质，在采取适当的实验预防措施，单时间点BLI可能是有用的非侵入性，高通量，定量评估肿瘤负荷。Mol Imaging（2004）3，117-124.
Bioluminescence imaging (BLI) is a highly sensitive tool for visualizing tumors, neoplastic development, metastatic spread, and response to therapy. Although BLI has engendered much excitement due to its apparent simplicity and ease of implementation, few rigorous studies have been presented to validate the measurements. Here, we characterize the nature of bioluminescence output from mice bearing subcutaneous luciferase-expressing tumors over a 4-week period. Following intraperitoneal or direct intratumoral administration of luciferin substrate, there was a highly dynamic kinetic profile of light emission. Although bioluminescence was subject to variability, strong correlations (r > .8, p < .001) between caliper measured tumor volumes and peak light signal, area under light signal curve and light emission at specific time points were determined. Moreover, the profile of tumor growth, as monitored with bioluminescence, closely resembled that for caliper measurements. The study shows that despite the dynamic and variable nature of bioluminescence, where appropriate experimental precautions are taken, single time point BLI may be useful for noninvasive, high-throughput, quantitative assessment of tumor burden. Mol Imaging (2004) 3, 117-124.