Constitutive heterologous overexpression of a TIR-NB-ARC-LRR gene encoding a putative disease resistance protein from wild Chinese Vitis pseudoreticulata in Arabidopsis and tobacco enhances resistance to phytopathogenic fungi and bacteria
Constitutive heterologous overexpression of a TIR-NB-ARC-LRR gene encoding a putative disease resistance protein from wild Chinese Vitis pseudoreticulata in Arabidopsis and tobacco enhances resistance to phytopathogenic fungi and bacteria
复制标题
拟南芥和烟草中编码来自中国野生葡萄的推定抗病蛋白的 TIR-NB-ARC-LRR 基因的组成型异源过度表达增强了对植物病原真菌和细菌的抗性
DOI：
10.1016/j.plaphy.2017.01.017
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发表时间：
2017
影响因子：
6.5
通讯作者：
Wang Xiping
中科院分区：
文献类型：
--
作者：
Wen Zhifeng;Yao Liping;Singer Stacy D.;Muhammad Hanif;Li Zhi;Wang Xiping
植物使用抗性（R）蛋白来检测病原体效应蛋白并激活它们针对病原体的先天免疫应答。这些蛋白质中的大多数含有NB-ARC（APAF-1、R蛋白和CED-4共享的核苷酸结合衔接子）结构域沿着富含亮氨酸的重复序列（LRR），并且一些还带有toll白细胞介素1受体（TIR）结构域。在本研究中，我们确定了一个基因编码的TIR-NB-ARC-LRR R蛋白（VpTNL 1）（GenBank登录号KX 649890）从野生中国葡萄藤葡萄登记“百合-35-1”，这是以前的转录组学分析与白粉病（PM; Erysiphenecator（Schw.）。在接种钩虫和水杨酸（SA）处理后，VpTNL 1的转录被高度诱导。序列分析表明，推导的氨基酸序列在N端含有一个TIR结构域，在C端含有沿着的NB-ARC和4个LRR结构域。VpTNL 1在拟南芥中的组成型表达导致野生型或矮化表型。有趣的是，表型正常的转基因株系显示出对拟南芥PM、Golovinomycescichoracearum以及对毒性细菌病原体假单胞菌pv.番茄DC 3000。同样，在烟草中组成型表达VpTNL 1被发现赋予增强的对烟草PM，白粉病菌DC的抗性。随后对VpTNL 1启动子的分离和缺失分析表明富含TC的重复序列和TCA元件可能分别在其对葡萄缩节线虫和SA处理的应答中起重要作用。综上所述，这些结果表明VpTNL 1有助于葡萄藤对PM的抗性，并为未来通过育种和/或生物技术改良葡萄提供了一个有趣的基因靶标。
Plants use resistance (R) proteins to detect pathogen effector proteins and activate their innate immune response against the pathogen. The majority of these proteins contain an NB-ARC (nucleotide-binding adaptor shared by APAF-1, R proteins, and CED-4) domain along with a leucine-rich repeat (LRR), and some also bear a toll interleukin 1 receptor (TIR) domain. In this study, we characterized a gene encoding a TIR-NB-ARC-LRR R protein (VpTNL1) (GenBank accession number KX649890) from wild Chinese grapevine Vitispseudoreticulataaccession “Baihe-35-1”, which was identified previously from a transcriptomic analysis of leaves inoculated with powdery mildew (PM; Erysiphenecator (Schw.)). TheVpTNL1transcript was found to be highly induced inV. pseudoreticulatafollowing inoculation withE. necator, as well as treatment with salicylic acid (SA). Sequence analysis demonstrated that the deduced amino acid sequence contained a TIR domain at the N-terminus, along with an NB-ARC and four LRRs domains within the C-terminus. Constitutive expression ofVpTNL1in Arabidopsis thaliana resulted in either a wild-type or dwarf phenotype. Intriguingly, the phenotypically normal transgenic lines displayed enhanced resistance to Arabidopsis PM,Golovinomycescichoracearum, as well as to the virulent bacterial pathogen Pseudomonas syringae pv. tomato DC3000. Similarly, constitutive expression ofVpTNL1in Nicotiana tabacum was found to confer enhanced resistance to tobacco PM,Erysiphe cichoacearumDC. Subsequent isolation of theVpTNL1promoter and deletion analysis indicated that TC-rich repeats and TCA elements likely play an important role in its response toE. necatorand SA treatment, respectively. Taken together, these results indicate thatVpTNL1contributes to PM resistance in grapevine and provide an interesting gene target for the future amelioration of grape via breeding and/or biotechnology.