Establishment of introgression libraries in hybrid rye (Secale cereale L.) from an Iranian primitive accession as a new tool for rye breeding and genomics
Establishment of introgression libraries in hybrid rye (Secale cereale L.) from an Iranian primitive accession as a new tool for rye breeding and genomics
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DOI：
10.1007/s00122-008-0808-1
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发表时间：
2008-08-01
影响因子：
5.4
通讯作者：
Geiger, H. H.
中科院分区：
文献类型：
--
作者：
Falke, K. C.;Susic, Z.;Geiger, H. H.
优良育种材料的遗传多样性可以通过外来种质的渐渗来增加，以确保长期的选择反应。我们的研究目的是开发和表征的前两个黑麦渐渗文库标记辅助回交产生的，并证明其潜在的应用，提高黑麦的烘焙品质。从自交系L2053-N（轮回亲本）和杂合伊朗原始群体Altevogt 14160（供体）之间的杂交开始，进行两个回交（BC）和三个自交世代以建立渐渗文库A和B。利用扩增片段长度多态性（AFLP（R）标记）和简单重复序列（SSR）对BC 1-BC 2S 3的候选渐渗系（pre-IL）进行筛选和表征。两个基因渗入文库各自包含40个BC 2S 3前IL。为了分析外来供体染色体片段（DCS）的表型效应，我们在2005年和2006年在四个地点的每个重复田间试验中评估了戊聚糖和淀粉含量的本身性能。渐渗文库A和B分别覆盖总供体基因组的74%和59%。前IL主要包含2 - 4个纯合DCS，平均长度为12.9 cM（A）和10.0 cM（B）。我们检测到8个（A）和9个（B）具有显著（P < 0.05）更高戊聚糖含量的前IL和2个（B）具有显著（P < 0.05）更高淀粉含量的前IL。因此，我们的研究结果表明，黑麦的外来遗传资源携带有利的等位基因的烘烤品质性状，可以利用标记辅助选择（MAS）的优良育种材料。这些渐渗文库可以大大促进黑麦育种计划，并提供了一个有前途的机会，进行功能基因组学。
Genetic diversity of elite breeding material can be increased by introgression of exotic germplasm to ensure long-term selection response. The objective of our study was to develop and characterize the first two rye introgression libraries generated by marker-assisted backcrossing and demonstrate their potential application for improving the baking quality of rye. Starting from a cross between inbred line L2053-N (recurrent parent) and a heterozygous Iranian primitive population Altevogt 14160 (donor) two backcross (BC) and three selfing generations were performed to establish introgression libraries A and B. Amplified fragment length polymorphisms (AFLP (R) markers) and simple sequences repeats (SSRs) were employed to select and characterize candidate introgression lines (pre-ILs) from BC1 to BC2S3. The two introgression libraries comprise each 40 BC2S3 pre-ILs. For analyzing the phenotypic effects of the exotic donor chromosome segment (DCS) we evaluated the per se performance for pentosan and starch content in replicated field trials at each of four locations in 2005 and 2006. Introgression library A and B cover 74 and 59% of the total donor genome, respectively. The pre-ILs contained mostly two to four homozygous DCS, with a mean length of 12.9 cM (A) and 10.0 cM (B). We detected eight (A) and nine (B) pre-ILs with a significant (P < 0.05) higher pentosan content and two pre-ILs (B) with a significant (P < 0.05) higher starch content than the elite recurrent parent. Thus, our results indicate that exotic genetic resources in rye carry favorable alleles for baking quality traits, which can be exploited for improving the elite breeding material by marker-assisted selection (MAS). These introgression libraries can substantially foster rye breeding programs and provide a promising opportunity to proceed towards functional genomics.