Gene for A-type inclusion body protein is useful for a polymerase chain reaction assay to differentiate orthopoxviruses
Gene for A-type inclusion body protein is useful for a polymerase chain reaction assay to differentiate orthopoxviruses
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DOI：
10.1016/s0166-0934(96)02155-6
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发表时间：
1997-03-01
影响因子：
3.1
通讯作者：
Esposito, JJ
中科院分区：
文献类型：
--
作者：
Meyer, H;Ropp, SL;Esposito, JJ
使用基于牛痘病毒嗜酸型包涵体 (ATI) 主要蛋白质成分编码序列的单引物对，通过聚合酶链式反应扩增基因组 DNA 来鉴定和区分正痘病毒种类。 8 种旧世界病毒（牛痘、天花、猴痘、骆驼痘、痘苗病毒和痘苗病毒）中的 6 种和 3 种新世界病毒（臭鼬痘、田鼠痘和浣熊痘）可用的 DNA 产生的扩增子大小范围为 510 至 1673 个碱基对，具体取决于物种，但浣熊痘病毒 DNA 不扩增。扩增子的 XbaI 消化凝胶电泳图谱提高了差异的分辨率。 (C) 1997 Elsevier Science B.V.
Orthopoxvirus species were identified and differentiated by polymerase chain reaction amplification of genome DNA using a single primer-pair based on sequences coding for the major protein component of the cowpox virus acidophilic-type inclusion body (ATI). DNA available for 6 of 8 Old World (cowpox, variola, monkeypox, camelpox, ectromelia and vaccinia viruses) and 3 New World (skunkpox, volepox, and raccoonpox) resulted in amplicons that ranged in size from 510 to 1673 base pairs depending on the species, except for raccoonpox virus DNA which did not amplify. XbaI digest gel electrophoresis profiles of the amplicons improved resolution of the differences. (C) 1997 Elsevier Science B.V.