Characterization of the anti-inflammatory Lactobacillus reuteri BM36301 and its probiotic benefits on aged mice.
Characterization of the anti-inflammatory Lactobacillus reuteri BM36301 and its probiotic benefits on aged mice.
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抗炎乳酸杆菌REUTERI BM36301及其对老年小鼠的益生菌益处的表征。
DOI：
10.1186/s12866-016-0686-7
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发表时间：
2016-04-19
期刊：
影响因子：
4.2
通讯作者：
Kim HS
中科院分区：
文献类型：
--
作者：
Lee J;Yang W;Hostetler A;Schultz N;Suckow MA;Stewart KL;Kim DD;Kim HS
肠道微生物群在宿主免疫调节中发挥着比最初预期更重要的作用。由于微生物的许多益处是高度菌株特异性的，其机制细节在很大程度上仍然难以捉摸，因此进一步鉴定具有免疫调节潜力的新型益生菌是非常有趣的。我们筛选了我们收集的益生菌乳酸菌（LAB）在调节宿主免疫反应方面的功效。当LAB培养上清液加入THP-1细胞时，一些LAB的特征是抑制TNF-α诱导，表明LAB具有抗炎潜力。这些抑制物质没有被热或胰蛋白酶灭活。另一方面，用活的细菌细胞直接处理THP-1发现了一组促炎的LAB，刺激了TNF-α的显著产生。其中，我们选择罗伊氏乳杆菌BM36301作为抗炎菌株，罗伊氏乳杆菌BM36304作为促炎菌株，进一步研究它们的体内作用。我们给C57BL/6小鼠提供了这些细菌的饮用水，同时给它们喂食标准饮食20周。有趣的是，这些罗伊氏乳杆菌菌株根据小鼠的性别引起了不同的结果。也就是说，接受抗炎BM36301治疗的男性体重增加较少，睾酮水平较高；经BM36301处理的雌性小鼠血清TNF-α水平较低，皮肤健康，毛囊生成和毛发生长活跃。此外，虽然用促炎BM36304治疗的男性血清中TNF-α和胰岛素水平较高，但相比之下，雌性没有受到这种细菌菌株的影响。选择罗伊氏乳杆菌BM36301作为体外抗炎菌株。它帮助老鼠在衰老时保持健康状态。这些发现表明罗伊氏乳杆菌BM36301是一种潜在的益生菌菌株，可以改善衰老问题的各个方面。本文的在线版本（doi:10.1186/s12866-016-0686-7）包含补充材料，可供授权用户使用。
The gut microbiota is playing more important roles in host immune regulation than was initially expected. Since many benefits of microbes are highly strain-specific and their mechanistic details remain largely elusive, further identification of new probiotic bacteria with immunoregulatory potentials is of great interest. We have screened our collection of probiotic lactic acid bacteria (LAB) for their efficacy in modulating host immune response. Some LAB are characterized by suppression of TNF-α induction when LAB culture supernatants are added to THP-1 cells, demonstrating the LAB’s anti-inflammatory potential. These suppressive materials were not inactivated by heat or trypsin. On the other hand, treatment of THP-1 directly with live bacterial cells identified a group of pro-inflammatory LAB, which stimulated significant production of TNF-α. Among those, we chose the Lactobacillus reuteri BM36301 as an anti-inflammatory strain and the L. reuteri BM36304 as a pro-inflammatory strain, and further studied their in vivo effects. We supplied C57BL/6 mice with these bacteria in drinking water while feeding them a standard diet for 20 weeks. Interestingly, these L. reuteri strains evoked different consequences depending on the gender of the mice. That is, males treated with anti-inflammatory BM36301 experienced less weight gain and higher testosterone level; females treated with BM36301 maintained lower serum TNF-α as well as healthy skin with active folliculogenesis and hair growth. Furthermore, while males treated with pro-inflammatory BM36304 developed higher serum levels of TNF-α and insulin, in contrast females did not experience such effects from this bacteria strain. The L. reuteri BM36301 was selected as an anti-inflammatory strain in vitro. It helped mice maintain healthy conditions as they aged. These findings propose the L. reuteri BM36301 as a potential probiotic strain to improve various aspects of aging issues. The online version of this article (doi:10.1186/s12866-016-0686-7) contains supplementary material, which is available to authorized users.