Isolation of an Insulin‐Responsive Preadipose Cell Line and a Mammary Tumor Virus‐Producing, Dome‐Forming Epithelial Cell Line from a Mouse Mammary Tumor
Isolation of an Insulin‐Responsive Preadipose Cell Line and a Mammary Tumor Virus‐Producing, Dome‐Forming Epithelial Cell Line from a Mouse Mammary Tumor
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从小鼠乳腺肿瘤中分离胰岛素反应性前脂肪细胞系和产生乳腺肿瘤病毒、形成圆顶的上皮细胞系
DOI：
10.1111/j.1440-169x.1984.00223.x
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发表时间：
1984
期刊：
影响因子：
4.6
通讯作者：
M. Koga
中科院分区：
文献类型：
--
作者：
J. Enami;Sonoko Enami;M. Koga
两个功能性组织培养细胞系，MTD和MTF细胞系，已从小鼠乳腺肿瘤中分离。MTD细胞是上皮细胞，并保留运输液体的能力，导致形成称为“圆顶”或“半囊”的三维充满液体的多细胞结构。MTD细胞的另一个特性是产生鼠乳腺肿瘤病毒（MTV）。通过免疫学、电泳和酶分析验证MTV释放到培养基中。在培养基中添加地塞米松可促进圆顶的形成和MTV的产生。因此，MTD细胞保留了原始乳腺肿瘤细胞的形态和功能特性。
Two functional tissue culture cell lines, MTD and MTF cell lines, have been isolated from a mouse mammary tumor. MTD cells are epithelial and retain the ability to transport fluid leading to the formation of three‐dimensional fluid‐filled multicellular structures called “domes” or “hemicysts”. Another property of MTD cells is the production of murine mammary tumor virus (MTV). Release of MTV into the culture medium was verified by immunological, electrophoretic and enzymatic analyses. Addition of dexamethasone in the culture medium enhanced both the formation of domes and the production of MTV. Thus, MTD cells retain the morphological and functional properties of the original mammary tumor cells.