Current‐voltage relationships in the crystalline lens.
Current‐voltage relationships in the crystalline lens.
复制标题
晶状体中的电流-电压关系。
DOI：
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发表时间：
1976
期刊：
影响因子：
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通讯作者：
J. Rae
中科院分区：
文献类型：
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作者：
R. Eisenberg;J. Rae
1.蛙眼晶体透镜细胞之间的电耦合使用两个微透镜电极进行研究，一个用于传递电流，一个用于记录电位。在大多数实验中，两个电极都放置在透镜后表面内部，距离表面约200 μ m的深度。施加电流的阶跃函数，并在许多不同的电极间隔处测量所产生的电压变化的时间过程。2.电压变化具有快速分量和慢速分量，快速分量仅局部地发生在靠近电流通过微电极的区域中，慢速分量在空间上是均匀的，与离电流微电极的距离无关。3.这种行为是由一个单一的大型球形细胞的电模型预测，因此该模型可以用来分析我们的数据。4.透镜“内部”的电阻率（细胞质和耦合电阻率）为625 Ω cm;透镜“膜”的电阻为2751 Ω cm 2。5.数据和分析有助于调和透镜的电特性的先前测量之间的差异，并且清楚地示出从电池到电池存在实质性的电耦合。该方法应允许在白内障形成的晶体透镜中的电耦合的作用的调查。
1. Electrical coupling between the cells of the crystalline lens of the frog eye was studied using two intralenticular micro‐electrodes, one to pass current and one to record potential. In most experiments, both electrodes were placed just inside the posterior surface of the lens at a depth of approximately 200 mum from the surface. Step functions of current were applied and the time course of the resulting change in voltage was measured at many different electrode separations. 2. The voltage change has both a fast component, which occurs only locally in the region close to the current passing micro‐electrode, and a slow component, which is spatially uniform, independent of distance from the current micro‐electrode. 3. This behaviour is predicted by an electrical model of a single large spherical cell, and so that model can be used to analyse our data. 4. The resistivity of the lens 'interior' (both cytoplasm and coupling resistivity) is 625 omega cm; the resistance of the lens 'membrane' is 2751 omega cm2. 5. The data and analysis help to reconcile discrepancies between previous measurements of the electrical properties of the lens and show clearly that there is substantial electrical coupling from cell to cell. The method should allow investigation of the role of electrical coupling in cataract formation in the crystalline lens.