Culture and transfer of human blastocysts increases implantation rates and reduces the need for multiple embryo transfers
Culture and transfer of human blastocysts increases implantation rates and reduces the need for multiple embryo transfers
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DOI：
10.1016/s0015-0282(97)00438-x
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发表时间：
1998-01-01
影响因子：
6.7
通讯作者：
Schoolcraft, WB
中科院分区：
文献类型：
--
作者：
Gardner, DK;Vella, P;Schoolcraft, WB
目的：探讨在序贯培养基中移植第5天的囊胚是否比第3天移植的囊胚着床率更高。设计：胚胎培养制度的比较研究。单位：私人执业辅助生殖技术中心。患者：23例接受常规IVF周期的患者。干预：在标准培养条件或无血清化学培养基中培养胚胎至第3天。随后，从第3天到第5天，在第二种专门设计用于支持囊胚发育的无血清培养基中培养101个胚胎。主要观察指标：第3天胚胎细胞数量和质量。第5天囊胚发育。着床率（由胎儿心脏决定）和持续妊娠率（PR）。结果：囊胚期移植的胚胎着床率是第3天（约8细胞期）移植的胚胎的两倍。与第5天相比，第3天需要更多的胚胎来建立相似的pr。结论：在没有共培养和血清的情况下，在序贯培养基中可以获得活的人囊胚。体外受精中囊胚的移植将促进高pr，同时限制移植的胚胎数量，从而将多胎妊娠的风险降至最低。(C) 1998年，美国生殖医学学会。
Objective: To determine whether the transfer of blastocysts on day 5, developed in sequential culture media, resulted in an increase in implantation rate compared with embryos transferred on day 3.Design: Comparative study of embryo culture regimes.Setting: Private practice assisted reproductive technology center.Patient(s): Twenty-three patients undergoing routine IVF cycles.Intervention(s): Culture of embryos to day 3 in either standard culture conditions or a serum-free chemically defined medium. One hundred one embryos were subsequently cultured from day 3 to day 5 in a second serum-free medium specifically designed to support development of the blastocyst.Main Outcome Measure(s): Embryo cell number and quality on day 3. Blastocyst development on day 5. Implantation rate (determined by fetal heart) and ongoing pregnancy rate (PR).Result(s): Implantation rates for embryos transferred at the blastocyst stage of development were twice that observed for embryos transferred on day 3, around the eight-cell stage. Significantly more embryos were required for transfer on day 3, compared with day 5, to establish similar PRs.Conclusion(s): Viable human blastocysts can be obtained in sequential culture media the in the absence of coculture and serum. Transfer of blastocysts in IVF will facilitate high PRs while limiting the number of embryos transferred and therefore minimizes the risk of multiple gestation. (C) 1998 by American Society for Reproductive Medicine.