The effect of pore size on tissue ingrowth and neovascularization in porous bioceramics of controlled architecture in vivo
The effect of pore size on tissue ingrowth and neovascularization in porous bioceramics of controlled architecture in vivo
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DOI：
10.1088/1748-6041/6/1/015007
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发表时间：
2011-02-01
影响因子：
4
通讯作者：
Dong Xin
中科院分区：
文献类型：
--
作者：
Bai Feng;Zhang Jinkang;Dong Xin
本研究旨在探讨在孔隙参数的精确控制下，孔隙大小对多孔生物陶瓷组织生长和新生血管形成的影响。为此，将具有4种不同大孔大小（300-400、400-500、500-600和600-700 μ m）但连接尺寸相同（120 μ m）且孔隙度不变的β -磷酸三钙（β - tcp）圆柱体植入兔腰背筋膜。对支架内形成的纤维组织和血管进行组织学和组织形态学观察。利用单光子发射计算机断层扫描（SPECT）对多孔生物陶瓷的血管化进行了分析。研究发现，孔隙大小作为多孔结构的重要参数，在多孔生物材料支架的组织浸润过程中起着重要作用。纤维组织的长入量随着孔隙大小的减小而增加。4种不同大孔尺寸（300-400、400-500、500-600、600-700 μ m）、固定连接尺寸为120 μ m的支架中，纤维组织面积（%）分别为60.5%、52.2%、41.3%、37.3%，在第4周显著降低（P < 0.01）。支架的孔径大小与大孔生物材料在体内植入后的新生血管形成密切相关。大的孔径有利于血管的生长，小于400 μ m的孔径限制了血管的生长，导致血管直径变小。
The purpose of this study was to investigate the role of pore size on tissue ingrowth and neovascularization in porous bioceramics under the accurate control of the pore parameters. For that purpose, beta-tricalcium phosphate (beta-TCP) cylinders with four different macropore sizes (300-400, 400-500, 500-600 and 600-700 mu m) but the same interconnection size (120 mu m) and unchangeable porosity were implanted into fascia lumbodorsalis in rabbits. The fibrous tissues and blood vessels formed in scaffolds were observed histologically and histomorphometrically. The vascularization of the porous bioceramics was analyzed by single-photon emission computed tomography (SPECT). It is found that pore size as an important parameter of a porous structure plays an important role in tissue infiltration into porous biomaterial scaffolds. The amount of fibrous tissue ingrowth increases with the decrease of the pore size. In four kinds of scaffolds with different macropore sizes (300-400, 400-500, 500-600 and 600-700 mu m) and a constant interconnection size of 120 mu m, the areas of fibrous tissue (%) were 60.5%, 52.2%, 41.3% and 37.3%, respectively, representing a significant decrease at 4 weeks (P < 0.01). The pore size of a scaffold is closely related to neovascularization of macroporous biomaterials implanted in vivo. A large pore size is beneficial for the growth of blood vessels, and the diameter of a pore smaller than 400 mu m limits the growth of blood vessels and results in a smaller blood vessel diameter.