The immediate early proteins of varicella-zoster virus.
The immediate early proteins of varicella-zoster virus.
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水痘带状疱疹病毒的早期蛋白质。
DOI：
10.1016/0042-6822(87)90423-5
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发表时间：
1987
期刊：
影响因子：
3.7
通讯作者：
Hyman,RW
中科院分区：
文献类型：
--
作者：
Shiraki,K;Hyman,RW
稳定、相对高滴度的水痘带状疱疹病毒 (VZV) 储液以及在近交系豚鼠中制备的高滴度抗 VZV 血清允许使用经典抑制剂方法鉴定 VZV 立即早期蛋白。 VZV 感染是在放线菌酮存在的情况下开始的。除去放线菌酮后，添加放线菌素 D 和放射性标记。标记期后，用抗 VZV 豚鼠血清对提取物进行免疫沉淀，并进行聚丙烯酰胺凝胶电泳和荧光照相或放射自显影。鉴定出四种摩尔重量分别为 185,000、69,000、43,000 和 34,000 的立即早期蛋白质。最大的三个是磷蛋白。
Stable, relatively high-titer varicella-zoster virus (VZV) stocks as well as a high-titer anti-VZV serum prepared in inbred guinea pigs have allowed the identification of VZV immediate early proteins using the classic inhibitor approach. VZV infection was initiated in the presence of cycloheximide. Following the removal of cycloheximide, actinomycin D and radiolabel were added. After the labeling period, extracts were immunoprecipitated with anti-VZV guinea pig serum and subjected to polyacrylamide gel electrophoresis and fluorography or autoradiography. Four immediate early proteins of mol wts 185,000, 69,000, 43,000, and 34,000 were identified. The largest three were phosphoproteins.