Bacterial lactase genes diversity in intestinal mucosa of dysbacterial diarrhea mice treated with Qiweibaizhu powder
Bacterial lactase genes diversity in intestinal mucosa of dysbacterial diarrhea mice treated with Qiweibaizhu powder
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七味白术散治疗菌群失调性腹泻小鼠肠黏膜细菌乳糖酶基因多样性
DOI：
10.1007/s13205-018-1460-3
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发表时间：
2018-09-27
期刊：
影响因子：
2.8
通讯作者：
Hui, Huaying
中科院分区：
文献类型：
--
作者：
Long, Chengxing;Liu, Yawei;Hui, Huaying
本研究旨在探讨七味白术散对肠道菌群失调性腹泻小鼠肠黏膜细菌乳糖酶基因的影响，探讨其治疗腹泻的作用机制。头孢拉定胶囊与硫酸庆大霉素混合液（23.33 mL/kg-1/d-1）灌胃5 d，建立细菌性腹泻小鼠模型。致泻成功后，治疗组小鼠灌胃QWBZP 3天。收集各组小鼠肠粘膜，采用Miseq宏基因组测序技术进行小鼠肠粘膜细菌乳糖酶基因多样性分析。结果显示，治疗组Chao 1、ACE、Simpson、Shannon指数均低于模型组，与对照组相似。从操作分类单位（OTU）获得了相同的结果。对照组、模型组和治疗组的OTU分别为298、435和254个。主成分分析（PCA）结果表明，正常组和治疗组的样本分布相对密集，个体间距离较小，而模型组则相反。此外，抗生素增加了细菌乳糖酶基因在门和属水平上的多样性和丰度。经QWBZP治疗后，上述指标均下降，与对照组相似。我们的研究结果表明，QWBZP对细菌乳糖酶基因多样性恢复到正常水平有积极的作用。此外，QWBZP还增加了溶藻和真核生物的丰度。
The current research tried to investigate the effect of Qiweibaizhu powder (QWBZP) on intestinal mucosa bacterial lactase gene from dysbacterial diarrhea mice, as the good therapeutic mechanism of QWBZP on antibiotics-induced diarrhea. Dysbacterial diarrhea mice model was constructed by gastric perfusion with mixture of cephradine capsules and gentamicin sulfate (23.33 mL kg−1day−1) for 5 days. After the success of establishing diarrhea, the mice in treatment group were gavaged with QWBZP for 3 days. Intestinal mucosa in each group was collected, and diversity of bacterial lactase genes in intestinal mucosa of mice was carried out by Miseq metagenome sequencing. The results showed the Chao1, ACE, Simpson and Shannon indices in treatment group were lower than model group and were similar to control group. The same result was obtained from the operational taxonomic units (OTUs). There were 298, 435 and 254 OTUs in the control group, model group and treatment group, respectively. Principal component analysis (PCA) indicated that samples distribution in both normal and treatment groups were relatively intensive, distances among individuals were small, while opposite results were observed in model group. Moreover, antibiotics increased the diversity and abundance of bacterial lactase genes at phylum and genus levels. However, they decreased and were similar to control group after treating with QWBZP. Our results indicate that QWBZP has a positive effect on the recovery of bacterial lactase gene diversity to normal level. In addition, QWBZP increase the abundance ofLysobacterandEukaryota.