Dual functional AgNPs-M13 phage composite serves as antibacterial film and sensing probe for monitoring the corrosion of chromium-containing dental alloys
Dual functional AgNPs-M13 phage composite serves as antibacterial film and sensing probe for monitoring the corrosion of chromium-containing dental alloys
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双功能AgNPs-M13噬菌体复合物作为抗菌膜和传感探针用于监测含铬牙科合金的腐蚀
DOI：
10.1016/j.cclet.2019.07.026
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发表时间：
2020-01-01
影响因子：
9.1
通讯作者：
Wang, Jianhua
中科院分区：
文献类型：
--
作者：
Yang, Ting;Li, Na;Wang, Jianhua
以微生物或植物组织为模板制备银纳米粒子（AgNPs）是一种绿色的制备方法，但存在收获率低、需要纯化等缺点。在此，我们提出了一个简单的协议，一锅制备银纳米粒子使用M13噬菌体作为生物模板，通过简单地混合硝酸银溶液与碱性M13噬菌体。在所获得的AgNPs-M13噬菌体复合物中，Cr（III）选择性地与噬菌体表面的氨基残基配位，并通过M13的桥连导致AgNPs聚集。这使得通过测量AgNP在600和405 nm处的吸光度比来进行Cr（III）的比色传感是可行的，这提供了14 nmol/L的LOD。该复合材料对革兰氏阳性菌和革兰氏阴性菌均表现出良好的杀菌活性，使其成为含铬牙科合金中有希望的抗菌膜，同时作为传感探针用于监测牙科合金的腐蚀。(C)2019中国化学会、中国医学科学院药物研究所。Elsevier B. V.出版，保留所有权利。
The preparation of silver nanoparticles (AgNPs) with microbe or plant tissues as bio-template offers green approach, while it suffers from low harvest and purification is needed. Herein, we propose a facile protocol for one-pot preparation of AgNPs using M13 phage as bio-template by simply mixing AgNO3 solution with alkali M13 phage. In the obtained AgNPs-M13 phage composite, Cr(III) selectively coordinates with the amino residues on phage surface and leads to the aggregation of AgNPs through the bridging of M13 phages. This makes it feasible for colorimetric sensing of Cr(III) by measuring the absorbance ratio of AgNPs at 600 and 405 nm, which provides a LOD of 14 nmol/L. The composite also showed favorable bactericidal activity for both Gram-positive and Gram-negative bacteria, making it a promising candidate as antibacterial film in chromium-containing dental alloys and meanwhile serve as a sensing probe for monitoring the corrosion of the dental alloys. (C) 2019 Chinese Chemical Society and Institute of Materia Medica, Chinese Academy of Medical Sciences. Published by Elsevier B.V. All rights reserved.