Metagenomics to paleogenomics: Large-scale sequencing of mammoth DNA
Metagenomics to paleogenomics: Large-scale sequencing of mammoth DNA
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DOI：
10.1126/science.1123360
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发表时间：
2006-01-20
期刊：
影响因子：
56.9
通讯作者：
Schuster, SC
中科院分区：
文献类型：
--
作者：
Poinar, HN;Schwarz, C;Schuster, SC
我们采用宏基因组学方法对来自西伯利亚的猛犸象（Mammuthus primigenius）样本的2800万碱基对的DNA进行了测序。由于样本保存得非常好，以及使用了最近开发的乳液聚合酶链反应和焦磷酸测序技术，测序读数中有1300万碱基对（45.4%）被确定为猛犸象的DNA。我们的数据与非洲象（Loxodonta africana）之间的序列一致性为98.55%，这与基于古生物学的500万到600万年的分化时间是相符的。该样本中环境DNA的多样性低得惊人。从这头猛犸象单个样本中可获取的内源DNA比例很高，这将使它的基因组得以完成，从而开启古基因组学领域。
We sequenced 28 million base pairs of DNA in a metagenomics approach, using a woolly mammoth (Mammuthus primigenius) sample from Siberia. As a result of exceptional sample preservation and the use of a recently developed emulsion polymerase chain reaction and pyrosequencing technique, 13 million base pairs (45.4%) of the sequencing reads were identified as mammoth DNA. Sequence identity between our data and African elephant (Loxodonta africana) was 98.55%, consistent with a paleontologically based divergence date of 5 to 6 million years. The sample includes a surprisingly small diversity of environmental DNAs. The high percentage of endogenous DNA recoverable from this single mammoth would allow for completion of its genome, unleashing the field of paleogenomics.