The characterization of intraepithelial lymphocytes, lamina propria leukocytes, and isolated lymphoid follicles in the large intestine of mice infected with the intestinal nematode parasite Trichuris muris
The characterization of intraepithelial lymphocytes, lamina propria leukocytes, and isolated lymphoid follicles in the large intestine of mice infected with the intestinal nematode parasite Trichuris muris
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DOI：
10.4049/jimmunol.175.10.6713
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发表时间：
2005-11-15
影响因子：
4.4
通讯作者：
Else, KJ
中科院分区：
文献类型：
--
作者：
Little, MC;Bell, LV;Else, KJ
尽管对细胞因子在寄生蠕虫免疫中的作用的了解越来越多，但最终导致蠕虫从大肠中排出的局部效应机制尚不清楚。我们用流式细胞术和免疫组织化学方法研究了耐药株和敏感株感染毛滴虫小鼠的大肠上皮内淋巴细胞(IEL)和固有层白细胞(LPL)的表型。感染后，白细胞聚集在上皮和固有层中，显示出不同品系小鼠之间的显著差异。在启动Th2应答的耐药小鼠中，浸润性CD4(+)、CD8(+)、B220(+)和F4/80(+)IEL和LPL的数量通常在蠕虫从肠道排出时最高，此时炎症以CD4(+)IEL和F4/80(+)LPL为主。相反，在启动Th1反应的易感小鼠中，IEL和LPL的数量增加得更缓慢，并在慢性感染后达到最高。此时以CD8(+)IEL和F4/80(+)LPL为主。因此，这项研究揭示了在耐药和敏感品系的小鼠中，驱虫或慢性感染持续存在的局部免疫反应。此外，我们第一次展示了大肠中孤立的淋巴滤泡，由B细胞和CD4(+)T细胞组成，中心区域有快速增殖的细胞。此外，我们还证明了这些结构的器官发生，以响应毛滴虫感染。
Despite a growing understanding of the role of cytokines in immunity to the parasitic helminth Trichuris muris, the local effector mechanism culminating in the expulsion of worms from the large intestine is not known. We used flow cytometry and immunohistochemistry to characterize the phenotype of large intestinal intraepithelial lymphocytes (IEL) and lamina propria leukocytes (LPL) from resistant and susceptible strains of mouse infected with T. muris. Leukocytes accumulated in the epithelium and lamina propria after infection, revealing marked differences between the different strains of mouse. In resistant mice, which mount a Th2 response, the number of infiltrating CD4(+), CD8(+), B220(+), and F4/80(+) IEL and LPL was generally highest around the time of worm expulsion from the gut, at which point the inflammation was dominated by CD4(+) IEL and F4/80(+) LPL. In contrast, in susceptible mice, which mount a Th1 response, the number of IEL and LPL increased more gradually and was highest after a chronic infection had developed. At this point, CD8(+) IEL and F4/80(+) LPL were predominant. Therefore, this study reveals the local immune responses underlying the expulsion of worms or the persistence of a chronic infection in resistant and susceptible strains of mouse, respectively. In addition, for the first time, we illustrate isolated lymphoid follicles in the large intestine, consisting of B cells interspersed with CD4(+) T cells and having a central zone of rapidly proliferating cells. Furthermore, we demonstrate the organogenesis of these structures in response to T. muris infection.