VEGF targeting in mesotheliomas using an interleukin-6 signal inhibitor based on adenovirus gene delivery.
VEGF targeting in mesotheliomas using an interleukin-6 signal inhibitor based on adenovirus gene delivery.
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DOI：
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发表时间：
2010-06
影响因子：
2
通讯作者：
Y. Adachi;N. Yoshio-Hoshino;C. Aoki;N. Nishimoto
中科院分区：
文献类型：
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作者：
Y. Adachi;N. Yoshio-Hoshino;C. Aoki;N. Nishimoto
BACKGROUND Malignant mesotheliomas reportedly secrete interleukin-6 (IL-6) which augments production of vascular endothelial growth factor (VEGF) from mesothelioma cells. We previously reported the development of a new receptor inhibitor of IL-6 (NRI) by genetically engineering tocilizumab, a humanized anti-IL-6 receptor monoclonal antibody. Since NRI is encoded on a single gene, it is easily applicable to a gene delivery system using virus vehicles. In this study, we report VEGF targeting through NRI expression based on adenovirus-mediated gene delivery in mesothelioma cells. MATERIALS AND METHODS We constructed an NRI expression vector in the context of a tropism-modified adenovirus vector that had enhanced infectivity in mesothelioma cells. RESULTS This virus effectively induced NRI secretion from mesothelioma cells. This virus infection also reduced the VEGF production in mesothelioma cells. CONCLUSION These results indicate that NRI shows potential as an agent in the treatment of mesotheliomas.
