A disulphide isomerase gene (PDI-V) from Haynaldia villosa contributes to powdery mildew resistance in common wheat.
A disulphide isomerase gene (PDI-V) from Haynaldia villosa contributes to powdery mildew resistance in common wheat.
复制标题
Haynaldia villosa 的二硫键异构酶基因 (PDI-V) 有助于普通小麦的白粉病抗性
DOI：
10.1038/srep24227
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发表时间：
2016-04-13
影响因子：
4.6
通讯作者：
Wang X
中科院分区：
文献类型：
--
作者：
Faheem M;Li Y;Arshad M;Jiangyue C;Jia Z;Wang Z;Xiao J;Wang H;Cao A;Xing L;Yu F;Zhang R;Xie Q;Wang X
在这项研究中，我们报道了小麦野生近缘簇毛麦PDI类基因在抗白粉病中的作用。PDI-V蛋白含有两个保守的硫氧还蛋白(Trx)活性结构域(a和a‘)和一个非活性结构域(B)。PDI-V与E3连接酶CMPG1-V蛋白相互作用，CMPG1-V蛋白是白粉病反应的正调控因子。PDI-V被CMPG1-V单泛素化，未检测到降解。PDI-V位于簇毛麦染色体5V上，编码内质网蛋白。BGT感染簇毛麦叶片可诱导PDI-V的表达。病毒诱导的杜氏锥虫PDI基因沉默簇毛属双二倍体对抗性造成了危害。在中等感病小麦品种扬麦158中，单细胞瞬时过表达PDI-V或含有活性TXR结构域a的截短版本降低了吸器指数。在扬麦158中高表达PDI-V的稳定转基因品系在苗期和成熟期均表现出较好的抗白粉病能力。相反，点突变的PDI-VC57A的过表达并没有增加扬麦158的抗性水平。以上结果表明，PDI-V在白粉病抗性中起着关键作用，保守的Trx结构域a对其功能至关重要。
In this study, we report the contribution of a PDI-like gene from wheat wild relative Haynaldia villosa in combating powdery mildew. PDI-V protein contains two conserved thioredoxin (TRX) active domains (a and a′) and an inactive domain (b). PDI-V interacted with E3 ligase CMPG1-V protein, which is a positive regulator of powdery mildew response. PDI-V was mono-ubiquitinated by CMPG1-V without degradation being detected. PDI-V was located on H. villosa chromosome 5V and encoded for a protein located in the endoplasmic reticulum. Bgt infection in leaves of H. villosa induced PDI-V expression. Virus induced gene silencing of PDIs in a T. durum-H. villosa amphiploid compromised the resistance. Single cell transient over-expression of PDI-V or a truncated version containing the active TXR domain a decreased the haustorial index in moderately susceptible wheat cultivar Yangmai 158. Stable transgenic lines over-expressing PDI-V in Yangmai 158 displayed improved powdery mildew resistance at both the seedling and adult stages. By contrast over-expression of point-mutated PDI-VC57A did not increase the level of resistance in Yangmai 158. The above results indicate a pivotal role of PDI-V in powdery mildew resistance and showed that conserved TRX domain a is critical for its function.