2 SUBSETS OF RAT LYMPHOCYTES-T DEFINED WITH MONOCLONAL-ANTIBODIES
2 SUBSETS OF RAT LYMPHOCYTES-T DEFINED WITH MONOCLONAL-ANTIBODIES
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DOI：
10.1002/eji.1830100807
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发表时间：
1980-01-01
影响因子：
5.4
通讯作者：
WILLIAMS, AF
中科院分区：
文献类型：
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作者：
BRIDEAU, RJ;CARTER, PB;WILLIAMS, AF
用大鼠胸腺细胞糖蛋白免疫小鼠，制备了一种新的识别大鼠外周血T细胞亚群的单克隆抗体。该抗体，命名为MRC-OX-8，标记所有未被先前描述的W3/25单克隆抗体标记的外周T细胞。没有发现外周T细胞结合两种抗体，90%的胸腺细胞被双重标记。先天性无胸腺裸鼠的胸导管淋巴细胞未被任一抗体标记;此类动物的脾脏含有W3/25+细胞和MRC-OX-8+细胞。这些脾细胞亚群没有重叠。使用荧光激活细胞分选仪分离结合MRC-OX-8抗体的细胞，建立介导各种T细胞功能的T细胞表型。结合目前的结果与先前发表的结果，表明为抗体应答提供帮助的细胞和介导移植物抗-宿主反应表型为W3/25+ MRC-OX-8。在F1宿主中抑制抗体形成的亲本T细胞被鉴定为W3/25- MRC-OX-8+。由这些抗体定义的大鼠T细胞亚群和那些在小鼠中确定的Ly系列的同种抗体之间的关系进行了讨论和大鼠W3/25+亚群和最近确定的人类T细胞亚群之间的比较。
A new monoclonal mouse antibody that recognizes a subset of rat peripheral T cells was prepared by immunizing mice with rat thymocyte glycoprotein. This antibody, designated MRC-OX-8, labels all peripheral T cells that are unlabeled by the previously described W3/25 monoclonal antibody. No peripheral T cells were found that bound both antibodies; 90% of thymocytes were doubly labeled. Thoracic duct lymphocytes of congenitally athymic nude rats were not labeled by either antibody; the spleens of such animals contained W3/25+ cells and MRC-OX-8+ cells. These splenocyte subpopulations did not overlap. Using the fluorescence-activated cell sorter to isolate cells binding MRC-OX-8 antibody, the phenotype of T cells mediating various T cell functions was established. Combining the present results with those published previously, it is shown that the cells providing help for antibody responses and those mediating graft-vs.-host reactions are phenotypically W3/25+ MRC-OX-8. Parental T cells that suppress antibody formation in F1 hosts were identified as W3/25- MRC-OX-8+. The relationship between the rat T cell subsets defined by these antibodies and those in the mouse identified by the Ly series of alloantibodies is discussed and a comparison made between the rat W3/25+ subset and a recently identified human T cell subset.