Chemical reaction detection of catechins and proanthocyanidins with 4-dimethylaminocinnamaldehyde
Chemical reaction detection of catechins and proanthocyanidins with 4-dimethylaminocinnamaldehyde
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4-二甲氨基肉桂醛化学反应检测儿茶素和原花青素
DOI：
10.1016/s0021-9673(01)93963-9
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发表时间：
1989
影响因子：
4.1
通讯作者：
D. Treutter
中科院分区：
文献类型：
--
作者：
D. Treutter
描述了一种柱后衍生化的高效液相色谱方法，该方法可以特异性检测植物和饮料粗提取物中的儿茶素和原花青素。在浓硫酸存在下，4-二甲氨基肉桂醛可用作选择性试剂。该试剂的优点是其与黄烷醇的缩合产物在约640 nm处显示最大吸光度。其他酚类、吲哚类和萜烯类的反应产物具有不同的吸光度或反应非常弱。与其他酚类和取代吲哚相比，(−)-表儿茶素的敏感性提高了 200–40 000 倍。对于萜烯，该系数的范围为 4000（对于芳香百里酚）到 2·106。
An high-performance liquid chromatographic method with post-column derivatization is described which allows the specific detection of catechins and proanthocyanidins in crude extracts from plants and beverages. In the presence of concentrated sulphuric acid, 4-dimethylaminocinnamaldehyde can be employed as a selective reagent. The advantage of the reagent is that its condensation products with flavanols show maximum absorbance at about 640 nm. Other phenols, indoles and terpenes give reaction products with different absorbances or react very weakly. A 200–40 000 fold sensitivity was found for (−)-epicatechin as compared to other phenols and substituted indoles. Concerning the terpenes, this factor ranges from 4000 (for the aromatic thymol) to 2 · 106.