Virus-assisted mapping of neural inputs to a feeding center in the hypothalamus
Virus-assisted mapping of neural inputs to a feeding center in the hypothalamus
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DOI：
10.1126/science.1056602
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发表时间：
2001-03-30
期刊：
影响因子：
56.9
通讯作者：
Friedman, JM
中科院分区：
文献类型：
--
作者：
DeFalco, J;Tomishima, M;Friedman, JM
我们报告了一种伪狂犬病病毒的发展，可用于从选定的神经元逆行追踪。该病毒编码一种绿色荧光蛋白标记物，仅在表达Cre重组酶的神经元和与最初感染细胞突触接触的神经元中复制。将病毒注射到只在表达神经肽Y或瘦素受体的神经元中表达Cre的小鼠弓状核中。大脑切片显示，这些神经元接受来自下丘脑其他区域、杏仁核、皮质和其他大脑区域的神经元的输入。这些数据表明，更高的皮质中枢调节下丘脑中的瘦素信号。这种神经追踪方法在其他复杂神经回路的研究中可能是有用的。
We report the development of a pseudorabies virus that can be used for retrograde tracing from selected neurons. This virus encodes a green fluorescent protein marker and replicates only in neurons that express the Cre recombinase and in neurons in synaptic contact with the originally infected cells. The virus was injected into the arcuate nucleus of mice that express Cre only in those neurons that express neuropeptide Y or the leptin receptor. Sectioning of the brains revealed that these neurons receive inputs from neurons in other regions of the hypothalamus, as well as the amygdala, cortex, and other brain regions. These data suggest that higher cortical centers modulate leptin signaling in the hypothalamus. This method of neural tracing may prove useful in studies of other complex neural circuits.