Reduction of selenite to elemental selenium by Enterobacter cloacae SLD1a-1
Reduction of selenite to elemental selenium by Enterobacter cloacae SLD1a-1
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DOI：
10.2134/jeq1998.00472425002700060005x
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发表时间：
1998-11-01
影响因子：
2.4
通讯作者：
Frankenberger, WT
中科院分区：
文献类型：
--
作者：
Dungan, RS;Frankenberger, WT
在洗涤过的细胞悬浮液中研究了兼性厌氧细菌阴沟肠杆菌菌株SLD 1a-1（ATCC-700258），以评估将亚硒酸盐（SeO 32-）还原为元素硒（Se-0）所需的最佳条件。使用葡萄糖（1.4mM）作为电子供体的阴沟肠杆菌在2.5小时内从溶液中除去79%的添加的SeO 32-（7.9 μ M）。最佳SeO 32-还原发生在pH值为6.5和温度为40摄氏度。碳水化合物来源阿拉伯糖，木糖和山梨糖被发现显着提高SeO 32-还原葡萄糖。SeO 32-在7.9 μ M的减少被抑制硝酸盐在1至100倍以上的水平，亚硝酸盐在5和10倍以上的水平，而亚硫酸盐在2至4倍以上的水平被发现刺激SeO 32-的减少。阴沟肠杆菌生长在含有NO3-作为唯一末端电子受体和乙酸盐作为电子供体的厌氧孵育平板上。使用SeO 32-作为终端电子受体在厌氧呼吸不支持增长，只能减少到硒-0时，NO3-存在。
The facultative anaerobic bacterium Enterobacter cloacae strain SLD1a-1 (ATCC-700258) was studied in washed cell suspensions to assess optimal conditions required for the reduction of selenite (SeO32-) to elemental selenium (Se-0). Enterobacter cloacae using glucose (1.4 mM) as an electron donor removed 79% of the added SeO32-(7.9 mu M) from solution in 2.5 h. Optimal SeO32- reduction occurred at a pH of 6.5 and a temperature of 40 degrees C. Carbohydrate sources arabinose, xylose, and sorbose were found to significantly enhance SeO32- reduction over that of glucose. The reduction of SeO32- at 7.9 mu M was inhibited by nitrate at levels 1 to 100 times greater, nitrite at levels 5 and 10 times greater, while sulfite at levels of two to four times greater was found to stimulate the reduction of SeO32-. Enterobacter cloacae grows on anaerobically incubated plates containing NO3- as the sole terminal electron acceptor and acetate as the electron donor. Use of SeO32- as the terminal electron acceptor during anaerobic respiration did not support growth and could only be reduced to Se-0 when NO3- was present.