High-density fiber-optic DNA random microsphere array
High-density fiber-optic DNA random microsphere array
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DOI：
10.1021/ac0008284
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发表时间：
2000-11-15
影响因子：
7.4
通讯作者：
Walt, DR
中科院分区：
文献类型：
--
作者：
Ferguson, JA;Steemers, FJ;Walt, DR
研制了一种高密度光纤DNA微阵列传感器，用于并行监测多个DNA序列。将DNA探针功能化的3.1 mm直径的微球随机分布在500 mm直径的光学成像光纤中蚀刻的孔阵列中制备微阵列。使用光学编码方案和定制的成像系统对微球进行配准。用荧光标记的DNA靶观察杂交结果，检测限为10 fM。对于纳摩尔目标浓度，杂交时间需要几秒钟，分析在几分钟内完成。
A high-density fiber-optic DNA microarray sensor was developed to monitor multiple DNA sequences in parallel. Microarrays were prepared by randomly distributing DNA probe-functionalized 3.1-mum-diameter microspheres in an array of wells etched in a 500-mum-diameter optical imaging fiber. Registration of the microspheres was performed using an optical encoding scheme and a custom-built imaging system. Hybridization was visualized using fluorescent-labeled DNA targets with a detection limit of 10 fM. Hybridization times of seconds are required for nanomolar target concentrations, and analysis is performed in minutes.