Sequence-specific fragmentation of macronuclear DNA in a holotrichous ciliate
Sequence-specific fragmentation of macronuclear DNA in a holotrichous ciliate
复制标题
海毛纤毛虫大核 DNA 的序列特异性片段化
DOI：
10.1016/0092-8674(81)90321-4
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发表时间：
1981
期刊：
影响因子：
64.5
通讯作者：
E. Blackburn
中科院分区：
文献类型：
--
作者：
A. Katzen;G. Cann;E. Blackburn
Macronuclear DNA from the protozoan G. chattoni, a holotrichous ciliate, was analyzed. Most, if not all, of the macronuclear DNA is subchromosomal, ranging in size from above 100 kb down to 2.1 kb, with molecules in the lower molecular weight range being resolvable by gel electrophoresis into reproducible, specific, discrete size classes. A prominent class of linear 9.3 kb molecules consists of single free rRNA genes. Upon denaturation and partial renaturation, a high percentage of total macronuclear DNA was found as single-stranded circles. Sequence analysis showed that a minimum of 38 tandem repeats of the sequence CCCCAA is present in inverted orientation at each end of most or all Glaucoma macronuclear DNA molecules, including the rDNA. This sequence must therefore be recognized during site-specific fragmentation of chromosomes in macronuclear development.
