The stringent response factor, RelA, positively regulates T6SS4 expression through the RovM/RovA pathway in Yersinia pseudotuberculosis
The stringent response factor, RelA, positively regulates T6SS4 expression through the RovM/RovA pathway in Yersinia pseudotuberculosis
复制标题
严格反应因子 RelA 通过 RovM/RovA 途径正向调节假结核耶尔森菌中 T6SS4 的表达
DOI：
10.1016/j.micres.2018.12.002
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发表时间：
2019
影响因子：
6.7
通讯作者：
Wang Yao
中科院分区：
文献类型：
--
作者：
Yang Xiaobing;Song Yunhong;Dai Qingyun;Zhang Hongyun;Song Li;Wang Zhuo;Pan Junfeng;Wang Yao
VI型分泌系统（T6SS）是一种广泛分布于革兰氏阴性细菌中的多功能分子机制。 T6SS 的活性受到多种机制的严格调控，包括群体感应 (QS)、铁浓度和转录调节因子。在这里，我们证明了严格的反应调节因子 RelA 有助于细菌对假结核耶尔森氏菌的多种环境应激产生抵抗力。我们还揭示了严格反应（SR）的应激抵抗功能部分是由一般应激反应T6SS4系统介导的。 RelA 正向调节 T6SS4 的表达，以对抗由 RovM 和 RovA 调节因子共同介导的营养饥饿所产生的各种应激。这些发现不仅揭示了T6SS4在SR诱导的应激抵抗中的重要作用，而且还揭示了调节T6SS4表达以应对饥饿应激的新途径。
The type VI secretion system (T6SS) is a versatile molecular machinery widely distributed in Gram-negative bacteria. The activity of the T6SS is tightly regulated by various mechanisms, including quorum sensing (QS), iron concentration, and transcriptional regulators. Here we demonstrated that the stringent response regulator, RelA, contributes to bacterial resistance to multiple environmental stresses inYersinia pseudotuberculosis. We also revealed that the stress resistance function of stringent response (SR) was partially mediated by the general stress response T6SS4 system. RelA positively regulates the expression of T6SS4 to combat various stresses in response to nutrition starvation collectively mediated by the RovM and RovA regulators. These findings revealed not only the important role of T6SS4 in SR induced stress resistance, but also a new pathway to regulate T6SS4 expression in response to starvation stress.