Cognate Th2-B Cell Interaction is Essential for the Autoantibody Production in Pemphigus Vulgaris
Cognate Th2-B Cell Interaction is Essential for the Autoantibody Production in Pemphigus Vulgaris
复制标题
同源 Th2-B 细胞相互作用对于寻常型天疱疮自身抗体的产生至关重要
DOI：
10.1007/s10875-011-9597-4
复制
发表时间：
2012-02-01
影响因子：
9.1
通讯作者：
Pan, Meng
中科院分区：
文献类型：
--
作者：
Zhu, Haiqin;Chen, Yayuan;Pan, Meng
Pemphigus vulgaris (PV) is a Th2-dominant autoimmune skin disease. We showed that indeed active PV patients had a biased Th2 response and specific IgG4 autoantibodies were dominant. To further investigate the role of antigen-specific Th2 cells in the regulation of pathogenic Dsg3-IgG antibody production, we used recombined Dsg3 protein to immunize wild-type C57BL/6 mice with aluminum hydroxide or complete Freund's adjuvant as adjuvant. CD4(+) T cells from Dsg3-immunized mice were adoptively transferred into TCR-beta chain deficient mice. The transferred CD4(+) T cells were readily seen in the peripheral blood and spleen, and interacted with B cells, resulting in B-cell activation. Furthermore, transferred CD4(+) T cells from mice immunized with Dsg3 plus Alum with Th2 phenotype were able to render unprimed B cells to secrete Dsg3-specific IgG1 antibody in vivo. Taken together, these results provide the first demonstration of direct role of Dsg3-reactive CD4(+) T (Th2) cells in the regulation of pathologic anti-Dsg3 antibody production.
