Molecular Identification and Antifungal Susceptibility of Yeast Isolates Causing Fungemia Collected in a Population-Based Study in Spain in 2010 and 2011
Molecular Identification and Antifungal Susceptibility of Yeast Isolates Causing Fungemia Collected in a Population-Based Study in Spain in 2010 and 2011
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DOI：
10.1128/aac.02155-13
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发表时间：
2014-03-01
影响因子：
4.9
通讯作者：
Cuenca-Estrella, Manuel
中科院分区：
文献类型：
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作者：
Guinea, Jesus;Zaragoza, Oscar;Cuenca-Estrella, Manuel
我们报告了在 29 家西班牙医院进行的 CANDIPOP 基于人群的研究中收集的引起真菌血症的分离株的分子鉴定和抗真菌敏感性。总共收集了 781 株分离株（来自 767 名患者，其中 14 名患者患有混合性真菌血症）。最常见的物种是白色念珠菌（44.6%）、近平滑念珠菌（24.5%）、光滑念珠菌（13.2%）、热带念珠菌（7.6%）、克柔念珠菌（1.9%）、季里蒙念珠菌（1.7%）和卢西塔尼亚念珠菌（1.3%）。其他念珠菌和非念珠菌种类约占分离株的 5%。隐秘物种的存在率很低。与之前在西班牙进行的研究结果相比，光滑念珠菌的出现频率有所增加。使用EUCAST和CLSI M27-A3参考程序进行抗真菌药敏试验；这两种方法具有可比性。氟康唑敏感分离株的比例为 80%，与之前的研究结果相比似乎有所下降，这主要是由于光滑念珠菌的出现频率较高所致。使用物种特异性断点和流行病学截止值，伏立康唑和泊沙康唑的体外耐药率较低（
We report the molecular identifications and antifungal susceptibilities of the isolates causing fungemia collected in the CANDIPOP population-based study conducted in 29 Spanish hospitals. A total of 781 isolates (from 767 patients, 14 of them having mixed fungemia) were collected. The species found most frequently were Candida albicans (44.6%), Candida parapsilosis (24.5%), Candida glabrata (13.2%), Candida tropicalis (7.6%), Candida krusei (1.9%), Candida guilliermondii (1.7%), and Candida lusitaniae (1.3%). Other Candida and non-Candida species accounted for approximately 5% of the isolates. The presence of cryptic species was low. Compared to findings of previous studies conducted in Spain, the frequency of C. glabrata has increased. Antifungal susceptibility testing was performed by using EUCAST and CLSI M27-A3 reference procedures; the two methods were comparable. The rate of fluconazole-susceptible isolates was 80%, which appears to be a decrease compared to findings of previous studies, explained mainly by the higher frequency of C. glabrata. Using the species-specific breakpoints and epidemiological cutoff values, the rate of voriconazole and posaconazole in vitro resistance was low (