M1-RNA, THE RNA SUBUNIT OF ESCHERICHIA-COLI RIBONUCLEASE-P, CAN UNDERGO A PH-SENSITIVE CONFORMATIONAL CHANGE
M1-RNA, THE RNA SUBUNIT OF ESCHERICHIA-COLI RIBONUCLEASE-P, CAN UNDERGO A PH-SENSITIVE CONFORMATIONAL CHANGE
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DOI：
10.1021/bi00354a002
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发表时间：
1986-03-25
期刊：
影响因子：
2.9
通讯作者：
GUERRIERTAKADA, C
中科院分区：
文献类型：
--
作者：
ALTMAN, S;GUERRIERTAKADA, C
大肠杆菌核糖核酸酶P的催化亚基M1 RNA经全细胞提取纯化后，显然必须经历构象变化才能发挥催化作用。这种构象变化的速度取决于在不同温度和pH下孵育的时间。.DELTA.E.dbldag.在pH为7.5时的转变温度约为36kcal/mol。在10~100 mM范围内，镁离子浓度对构象变化不敏感。在孵育期内，观察到溶液中M1 RNA的A260降低，这加剧了30度的构象变化。C，但还不能与具体的结构调整直接相关。
After purification from extracts of whole cells, M1 RNA, the catalytic subunit of ribonuclease P from Escherichia coli, apparently must undergo a change in conformation before it can function catalytically. The rate of this conformational change is dependent upon the duration of incubation at various temperatures and pH. .DELTA.E.dbldag. of the transition at pH 7.5 is approximately 36 kcal/mol. The change in conformation is not sensitive to Mg2+ concentration between 10 and 100 mM. A decrease in A260 of M1 RNA in solution has been observed during the incubation period that potentiates the conformational change at 30.degree. C, but no direct correlation can yet be made to specific structural rearrangements.