PHOTOSENSITIZATION TISSUE MODEL
PHOTOSENSITIZATION TISSUE MODEL
复制标题
光敏化组织模型
DOI：
10.1111/j.1751-1097.1989.tb09186.x
复制
发表时间：
1989
影响因子：
3.3
通讯作者：
L. Grossweiner
中科院分区：
文献类型：
--
作者：
M. J. Schifano;L. Grossweiner
翻译后摘要-光敏化在光散射矩阵进行了研究与组织模型组成的聚苯乙烯微球散射颗粒，在存在的Photofrin II作为光敏剂，和枯草杆菌蛋白酶嘉士伯作为酶的目标。在不同的微球浓度下测量了在435 nm、545 nm和红光下照射的光动力学速率常数。反应速率几乎与微球无关，排除了光散射对综合光敏效率的显著影响。用一维扩散近似建模表明，增加散射体浓度导致较低的透射率和较高的漫反射，使得吸收分数几乎恒定。光散射系统中酶失活的量子效率为0.0014 ± 0.0003。在入射剂量方面，红光和绿色光具有大致相同的有效性，蓝光的效率高出两倍。
Abstract— Photosensitization in a light scattering matrix was investigated with a tissue model consisting of polystyrene microsphere scattering particles, in the presence of a Photofrin II as a photosensitizing agent, and subtilisin Carlsberg as an enzyme target. The photodynamic rate constant for irradiation at 435 nm, 545 nm, and red light was measured at different microsphere concentrations. The reaction rate was almost independent of the microspheres, ruling out a significant effect of light scattering on the integrated photosensitization efficiency. Modeling with the one‐dimensional diffusion approximation showed that increasing scatterer concentrations led to lower transmission and higher diffuse reflection, such that the fractional absorption was almost constant. The quantum efficiency of enzyme inactivation in the light‐scattering systems was 0.0014 ± 0.0003. In terms of incident dose, red and green light had approximately the same effectiveness and blue light was two‐fold more efficient.