Early Life Status Epilepticus and Stress Have Distinct and Sex-Specific Effects on Learning, Subsequent Seizure Outcomes, Including Anticonvulsant Response to Phenobarbital
Early Life Status Epilepticus and Stress Have Distinct and Sex-Specific Effects on Learning, Subsequent Seizure Outcomes, Including Anticonvulsant Response to Phenobarbital
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DOI：
10.1111/cns.12335
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发表时间：
2015-02-01
影响因子：
5.5
通讯作者：
Galanopoulou, Aristea S.
中科院分区：
文献类型：
--
作者：
Akman, Ozlem;Moshe, Solomon L.;Galanopoulou, Aristea S.
新生儿癫痫持续状态（SE）常与不良认知和癫痫结局相关。我们调查了三个事件的红藻氨酸诱导的SE（3 KA-SE）和母亲分离在未成年大鼠随后的学习，癫痫发作的敏感性，和后果，和苯巴比妥的抗惊厥作用，根据性别，类型和年龄在早期生活（EL）events.Methods3KA-SE或母亲分离诱导出生后的日子（PN）4-6或14-16。大鼠在巴恩斯迷宫（PN 16 -19）、锂-匹鲁卡品SE（PN 19）或氟乙基癫痫发作（PN 32）上进行测试。测试了苯巴比妥（20或40 mg/kg/大鼠，腹腔注射）预处理对氟甲基癫痫发作的抗惊厥作用。FluoroJadeB染色评估海马injuries. Results 3 KA-SE或分离PN 4 -6造成更多的男性短暂的学习延迟，并没有改变锂-匹鲁卡品SE lavenance，但加重其结果在女性。苯巴比妥的抗惊厥作用被保存和增强在特定的群体，根据性别，类型和年龄在EL event.ConclusionsEarly生活3 KA-SE和母亲分离导致更多，但短暂的认知功能障碍的男性，但加剧了随后的锂-匹鲁卡品SE在女性的后果。相比之下，在氟乙烯癫痫发作，EL事件显示出有益或无影响，这取决于性别，类型和年龄EL事件。
AimsNeonatal status epilepticus (SE) is often associated with adverse cognitive and epilepsy outcomes. We investigate the effects of three episodes of kainic acid-induced SE (3KA-SE) and maternal separation in immature rats on subsequent learning, seizure susceptibility, and consequences, and the anticonvulsant effects of phenobarbital, according to sex, type, and age at early life (EL) event.Methods3KA-SE or maternal separation was induced on postnatal days (PN) 4-6 or 14-16. Rats were tested on Barnes maze (PN16-19), or lithium-pilocarpine SE (PN19) or flurothyl seizures (PN32). The anticonvulsant effects of phenobarbital (20 or 40mg/kg/rat, intraperitoneally) pretreatment were tested on flurothyl seizures. FluoroJadeB staining assessed hippocampal injury.Results3KA-SE or separation on PN4-6 caused more transient learning delays in males and did not alter lithium-pilocarpine SE latencies, but aggravated its outcomes in females. Anticonvulsant effects of phenobarbital were preserved and potentiated in specific groups depending on sex, type, and age at EL event.ConclusionsEarly life 3KA-SE and maternal separation cause more but transient cognitive deficits in males but aggravate the consequences of subsequent lithium-pilocarpine SE in females. In contrast, on flurothyl seizures, EL events showed either beneficial or no effect, depending on gender, type, and age at EL events.