Comparative study of biohydrogen production by four dark fermentative bacteria
Comparative study of biohydrogen production by four dark fermentative bacteria
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DOI：
10.1016/j.ijhydene.2013.03.131
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发表时间：
2013-11
影响因子：
7.2
通讯作者：
C. Hu;A. Giannis;Chia-Lung Chen;W. Qi;Jing‐Yuan Wang
中科院分区：
文献类型：
--
作者：
C. Hu;A. Giannis;Chia-Lung Chen;W. Qi;Jing‐Yuan Wang
暗发酵由于其在无光源条件下产氢率高、底物种类多而成为一种很有前途的生物制氢方法。在厌氧条件下，研究了丁酸梭菌、巴氏梭菌、拜氏梭菌和产气肠杆菌4种暗发酵细菌以葡萄糖为底物的产氢潜力。通过间歇实验研究了葡萄糖初始浓度对产氢率、产氢速率和流出液中挥发性脂肪酸（VFA）浓度的影响。在四种不同的发酵菌中，C.丁酸菌在葡萄糖浓度为10 g/L时，产氢速率为18.29 mL-H2/L-培养基/h，比产氢速率为3.90 mL-H2/g-生物量/h。此外，还发现挥发性脂肪酸在不同发酵菌中的分布不同。丁酸和C. pasteuripudi的乙酸/丁酸比高于其他两种菌，有利于产氢。
Dark fermentation is a promising biological method for hydrogen production because of its high production rate in the absence of light source and variety of the substrates. In this study, hydrogen production potential of four dark fermentative bacteria (Clostridium butyricum,Clostridium pasteurianum,Clostridium beijerinckii, andEnterobacter aerogenes) using glucose as substrate was investigated under anaerobic conditions. Batch experiments were conducted to study the effects of initial glucose concentration on hydrogen yield, hydrogen production rate and concentration of volatile fatty acids (VFA) in the effluents. Among the four different fermentative bacteria,C. butyricumshowed great performance at 10 g/L of glucose with hydrogen production rate of 18.29 mL-H2/L-medium/hand specific hydrogen production rate of 3.90 mL-H2/g-biomass/h. In addition, it was found that the distribution of volatile fatty acids was different among the fermentative bacteria.C. butyricumandC. pasteurianumhad higher ratio of acetate to butyrate compared to the other two species, which favored hydrogen generation.