Size increase induced in Walker ascites cells by chemotactic factors.
Size increase induced in Walker ascites cells by chemotactic factors.
复制标题
趋化因子诱导沃克腹水细胞体积增大。
DOI：
10.1016/0304-3835(80)90023-3
复制
发表时间：
1980
期刊：
影响因子：
9.7
通讯作者：
P. Ward
中科院分区：
文献类型：
--
作者：
J. Wass;J. Varani;P. Ward
通过库尔特计数器测量，当与源自酶活性血清的肿瘤细胞趋化因子或与合成的趋化因子甲酰基蛋氨酸-亮氨酸-苯丙氨酸（f-met-leu-phe）相互作用时，悬浮中的Walker 256癌肉瘤细胞数量下降。库尔特计数仪计数的细胞数量下降的同时，也可能是由于平均细胞体积约为。20%。在肿瘤细胞中加入2-脱氧-d-葡萄糖以及趋化因子可以阻断这些作用。诱导这些反应的趋化因子的剂量与博伊登室试验中显示的诱导趋化反应的剂量密切相关。这些发现表明，在Boyden室试验中，非白细胞，如白细胞，在与适当的趋化因子相互作用时，除了趋化迁移外，还能产生其他反应。
Upon interaction with the tumor cell chemotactic factor derived from zymosan-activated serum or with the synthetic chemotactic factor, formylmethionyl-leucyl-phenylalanine (f-met-leu-phe), the number of Walker 256 carcinosarcoma cells in suspension dropped as measured by a Coulter Counter. The drop in the number of cells counted by the Coulter Counter was accompanied by and possibly attributed to a swelling in the mean cell volume of approx. 20%. These effects were blocked by the addition of 2-deoxy-d-glucose, along with the chemotactic factors, to the tumor cells. The doses of the chemotactic factors which induced these responses paralleled closely the doses which have been shown to induce a chemotactic response as measured in the Boyden Chamber assay. These findings indicate that non-leukocytic cells, like leukocytes, are capable of other responses in addition to chemotactic migration in the Boyden chamber assay upon interaction with appropriate chemotactic factors.