Very low density lipoproteins and lipoprotein lipase in serum of rats deficient in essential fatty acids.
Very low density lipoproteins and lipoprotein lipase in serum of rats deficient in essential fatty acids.
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缺乏必需脂肪酸的大鼠血清中的极低密度脂蛋白和脂蛋白脂肪酶。
DOI：
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发表时间：
1972
影响因子：
6.5
通讯作者：
F. D. Collins
中科院分区：
文献类型：
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作者：
G. G. De Pury;F. D. Collins
喂食缺乏必需脂肪酸的饮食的大鼠具有低水平的血清极低密度脂蛋白（VLDL）。结果发现，在腹腔注射肝素后，缺乏大鼠血浆中的脂蛋白脂酶活性水平高于正常大鼠。从正常和缺乏大鼠血清中分离的VLDL作为底物的大鼠血浆肝素后脂肪酶进行了比较。两种脂蛋白制剂的V（max）无显著差异，但缺陷动物脂蛋白的表观K（m）显著低于正常动物。这些观察结果表明，缺陷大鼠中VLDL的低浓度可以解释为（a）这些动物组织中脂蛋白脂肪酶活性增加和（B）缺陷大鼠的VLDL在低浓度下比正常大鼠的VLDL更快地被脂蛋白脂肪酶水解。
Rats fed a diet deficient in essential fatty acids have a low level of serum very low density lipoproteins (VLDL). It was found that after intraperitoneal injection of heparin, deficient rats had a higher level of lipoprotein lipase activity in their plasma than did normal rats. VLDL isolated from serum of normal and deficient rats were compared as substrates for postheparin lipase of rat plasma. There was no significant difference in V(max) between the two preparations of lipoproteins, but the apparent K(m) for lipoproteins from deficient animals was significantly less than that for normal animals. These observations suggest that the low concentration of VLDL in deficient rats may be explained (a) by an increased activity of lipoprotein lipase in the tissues of these animals and (b) by the VLDL of deficient rats being more rapidly hydrolyzed at low concentrations by lipoprotein lipase than VLDL from normal rats.