Functional characterisation of a putative rhamnogalacturonan II specific xylosyltransferase
Functional characterisation of a putative rhamnogalacturonan II specific xylosyltransferase
复制标题
DOI：
10.1016/j.febslet.2008.08.015
复制
发表时间：
2008-09-22
期刊：
影响因子：
3.5
通讯作者：
Petersen, Bent Larsen
中科院分区：
文献类型：
--
作者：
Egelund, Jack;Damager, Iben;Petersen, Bent Larsen
拟南芥基因At1g56550在毕赤酵母中表达，重组蛋白可催化d -木糖从udp - α - d -木糖转移到甲基α - l - focuside上。形成的产物经1D和2D H-1 NMR证实为Me α - d - xyl -(1,3)- α - l- fuc，与鼠李糖半乳酪酸II的a链目标结构相同。化学合成的由2-、3-或4位甲基衍生的甲基L- focuside作为受体底物进行了测试，但只有甲基4- o -甲基-L-fucopyranoside作为受体，尽管程度低于甲基-L- focuside。At1g56550被认为编码鼠李糖半乳糖酸II特异性木糖基转移酶。(c) 2008年欧洲生化学会联合会。Elsevier B.V.版权所有。
An Arabidopsis thaliana gene, At1g56550, was expressed in Pichia pastoris and the recombinant protein was shown to catalyse transfer of D-xylose from UDP-alpha-D-xylose onto methyl alpha-L-fucoside. The product formed was shown by 1D and 2D H-1 NMR spectroscopy to be Me alpha-D-Xyl-(1,3)-alpha-L-Fuc, which is identical to the proposed target structure in the A-chain of rhamnogalacturonan II. Chemically synthesized methyl L-fucosides derivatized by methyl groups on either the 2-, 3- or 4 position were tested as acceptor substrates but only methyl 4-O-methyl-alpha-L-fucopyranoside acted as an acceptor, although to a lesser extent than methyl alpha-L-fucoside. At1g56550 is suggested to encode a rhamnogalacturonan II specific xylosyltransferase. (c) 2008 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.