Determination of the carboxyl termini of proteins with ammonium thiocyanate and acetic anhydride, with direct identification of the thiohydantoins.
Determination of the carboxyl termini of proteins with ammonium thiocyanate and acetic anhydride, with direct identification of the thiohydantoins.
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DOI：
10.1021/bi00840a012
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发表时间：
1969-12
期刊：
影响因子：
2.9
通讯作者：
L. Cromwell;G. Stark
中科院分区：
文献类型：
--
作者：
L. Cromwell;G. Stark
劳伦斯·D克伦威尔和乔治R.斯塔克摘要：先前描述的用于肽的顺序降解的方法已经扩展到蛋白质的羧基末端的测定。用硫氰酸铵和过量的乙酸酐将蛋白质转化为蛋白酰硫代乙内酰脲可以通过使用六氟丙酮和乙酸的混合物作为溶剂在均匀溶液中进行。在通过凝胶过滤与过量试剂分离后，在12mHCl中裂解蛋白酰硫代乙内酰脲。
Laurence D. Cromwellf and George R. Stark abstract: The method described previously for sequential degradation of peptides has been extended to the determination of the carboxyltermini of proteins. Conversion of a protein into a proteinylthiohydantoin with ammonium thiocyanate and excess acetic anhydride can be carried out in homogeneous solution by using a mixture of hexafluoroacetone and acetic acid as the solvent. After separation from excess reagents by gel filtration, the proteinylthiohydantoin is cleaved in 12 m HC1.