Role of the donor nuclei in cloning efficiency: can the ooplasm reprogram any nucleus?
Role of the donor nuclei in cloning efficiency: can the ooplasm reprogram any nucleus?
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DOI：
10.1387/ijdb.103203yk
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发表时间：
2010-01-01
影响因子：
0.7
通讯作者：
Tsunoda, Yukio
中科院分区：
文献类型：
--
作者：
Kato, Yoko;Tsunoda, Yukio
自1997年绵羊体细胞核移植（SCNT）成功以来，克隆效率并没有显著提高。SCNT胚胎移植效率低的原因可能与供体核在卵质中的重编程不足有关。已经阐明，在SCNT胚胎中甲基化和乙酰化状态是无序的，并且与受精胚胎相比，在SCNT胚胎中基因表达模式是不同的且广泛变化的。本文着重讨论了供体细胞核在克隆效率中的作用，并讨论了卵质是否可以对任何细胞核进行重编程。
Cloning efficiency has not been dramatically improved after the first success of somatic cell nuclear transfer (SCNT) in sheep in 1997. The reasons for the low efficiency of SCNT embryos must be attributed to the insufficient reprogramming of the donor nucleus in ooplasm. It has been clarified that the methylation and acetylation status are disordered in SCNT embryos and the gene expression pattern is different and widely varied in SCNT embryos, compared with fertilized embryos. In this paper, we focused on the role of the donor nuclei in cloning efficiency, and discuss whether ooplasm can reprogram any nucleus.