Quantitative proteomic analyses reveal that RBBI3.3, a trypsin inhibitor protein, plays an important role in Magnaporthe oryzae infection in rice
Quantitative proteomic analyses reveal that RBBI3.3, a trypsin inhibitor protein, plays an important role in Magnaporthe oryzae infection in rice
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定量蛋白质组学分析表明，胰蛋白酶抑制剂蛋白 RBBI3.3 在水稻稻瘟病感染中发挥重要作用
DOI：
10.1007/s10725-018-0435-z
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发表时间：
2018-12-01
影响因子：
4.2
通讯作者：
Liang, Jiansheng
中科院分区：
文献类型：
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作者：
Cui, Shujian;Wang, Jiaoyu;Liang, Jiansheng
稻瘟病（M. oryzae）是稻瘟病的病原体，稻瘟病是中国最具破坏性的水稻病害。本研究旨在利用同量异位标签相对和绝对定量（iTRAQ）结合高通量质谱鉴定平台，明确水稻对米霉感染反应的分子机制，以促进优质抗病水稻新品种的选育。以抗性水稻品种古美2号和非抗性水稻品种丽江新团黑谷（LTH）构建了米曲霉感染模型。结果显示，总共鉴定出 1541 个蛋白质，其中 843 个蛋白质在两次生物重复分析之间重叠。七十一种蛋白质被归类为真菌反应性蛋白质。京都基因和基因组百科全书 (KEGG) 通路富集分析揭示了这些蛋白质在代谢过程中的重要作用。详细的表型分析表明，胰蛋白酶抑制剂 RBBI3.3 可有效抑制附着胞的初始形成。我们的定量蛋白质组学研究为了解不亲和水稻 Gumei2 中米霉抗性的分子机制提供了见解。 RBBI3.3作为关键防御调节因子的确定凸显了疾病控制的新可能性。
Magnaporthe oryzae (M. oryzae) is the causative agent of rice blast, the most destructive rice disease in China. This study was designed to ascertain the molecular mechanisms of the response of rice to M. oryzae infection to facilitate the breeding of new high-quality and disease-resistant rice varieties using isobaric tags for relative and absolute quantification (iTRAQ) combined with a high-throughput mass spectrometry identification platform. M. oryzae infection models were constructed with the resistant rice cultivar Gumei2 and the non-resistant cultivar Lijiangxintuanheigu (LTH). The results showed that total of 1541 proteins were identified, among which 843 proteins were overlapping between the two biological replicates analyses. Seventy-one proteins were classified as fungi-responsive. Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analyses revealed the important roles of these proteins in metabolic processes. Detailed phenotypic analyses revealed that the trypsin inhibitor RBBI3.3 was effective in inhibiting the initial formation of appressoria. Our quantitative proteomic study provides insights into the molecular mechanism underlying M. oryzae resistance in the incompatible rice Gumei2. The identification of RBBI3.3 as a key defense regulator highlights a new possibility for disease control.