Acute respiratory infection with mouse adenovirus type 1
Acute respiratory infection with mouse adenovirus type 1
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DOI：
10.1016/j.virol.2005.06.021
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发表时间：
2005-09-30
期刊：
影响因子：
3.7
通讯作者：
Spindler, KR
中科院分区：
文献类型：
--
作者：
Weinberg, JB;Stempfle, GS;Spindler, KR
对腺病毒呼吸道疾病发病机制的研究受腺病毒严格的种属特异性限制。用小鼠腺病毒1型(MAV-1)鼻腔接种成年C57BL/6小鼠，于感染后7d检测MAV-1早期区3(0)和六邻体基因在肺组织中的表达。7dpi时在呼吸道上皮细胞中检测到MAV-1 E3蛋白。在14dpi时未检测到病毒的mRNA或蛋白，但在21dpi时检测到MAV-1DNA。感染小鼠肺部的趋化因子转录水平在7dpi至14dpi之间增加。MAV-1感染后7dpi和14dpi肺组织出现斑片状细胞浸润。这是首次报道MAV-1在感染小鼠的呼吸道上皮细胞中的存在，并描述了MAV-1呼吸道感染引起的肺内趋化因子反应。小鼠MAV-1感染有可能成为人腺病毒呼吸道疾病炎症变化的模型。(C)2005 Elsevier Inc.保留所有权利。
Studies of the pathogenesis of adenovirus respiratory disease are limited by the strict species-specificity of the adenoviruses. Following intranasal inoculation of adult C57BL/6 mice with mouse adenovirus type 1 (MAV-1), we detected MAV-1 early region 3 (0) and hexon gene expression in the lungs at 7 days post-infection (dpi). We detected MAV-1 E3 protein in the respiratory epithelium at 7 dpi. We did not detect viral mRNA or protein at 14 dpi, but MAV-1 DNA was detected by PCR at 21 dpi. Chemokine transcript levels increased between 7 and 14 dpi in the lungs of infected mice. MAV-1 infection induced a patchy cellular infiltrate in lungs at 7 and 14 dpi. This is the first report demonstrating the presence of MAV-1 in the respiratory epithelium of infected mice and describing chemokine responses in the lung induced by MAV-1 respiratory infection. MAV-1 infection of mice has the potential to serve as a model for inflammatory changes seen in human adenovirus respiratory disease. (c) 2005 Elsevier Inc. All rights reserved.