High-Throughput Sequencing Characterizes Intertidal Meiofaunal Communities in Northern Gulf of Mexico (Dauphin Island and Mobile Bay, Alabama)
High-Throughput Sequencing Characterizes Intertidal Meiofaunal Communities in Northern Gulf of Mexico (Dauphin Island and Mobile Bay, Alabama)
复制标题
DOI：
10.1086/bblv227n2p161
复制
发表时间：
2014-10-01
影响因子：
1.6
通讯作者：
Halanych, Kenneth M.
中科院分区：
文献类型：
--
作者：
Brannock, Pamela M.;Waits, Damien S.;Halanych, Kenneth M.
小型底栖动物是食物网的重要组成部分，也是底栖动物和水柱之间进行营养交换的重要组成部分。由于深水地平线漏油事件(DWHOS)的潜在影响，最近的研究重点放在墨西哥湾的这些社区。特别是，阿拉巴马州莫比尔湾和Dauphin岛的潮间带小型底栖动物群落先前被证明从DWHOS之前的后生动物为主转变为泄漏后以真菌为主的群落。然而，关于这些社区内的可变性的知识仍然未知。在这里，我们使用Illumina高通量扩增子测序来检查在一年中注意到生物体转移的相同位置的变化。每两个月采集一次沉积物样本，为期一年(2011年7月至2012年7月)，通过测序18S rRNA基因的真核高变区V9来检查小型底栖动物群落。结果表明，真菌类群在这些群落中的存在是有限的，这表明以前报道的DWHOS对小型动物的急性影响显然是短期的。然而，与泄漏前的样品相比，这些小型底栖动物群落的后生动物分类群比例发生了变化。这种变化是由于漏油的长期影响，还是由于群落组成的变化，目前尚不清楚。在整个研究过程中，观察到了采样地点内和采样地点之间的分类学差异，这表明群落可能存在年度差异。在更长的时间范围内继续采样将更全面地了解这些群落内的季节性和变异性。需要这样的基线来评估未来的人为影响。
Meiofauna are important components of food webs and for nutrient exchange between the benthos and water column. Recent studies have focused on these communities in the Gulf of Mexico due to potential impacts of the Deepwater Horizon Oil Spill (DWHOS). In particular, intertidal meiofaunal communities from Mobile Bay and Dauphin Island, Alabama, were previously shown to shift from predominately metazoan taxa prior to DWHOS to a fungal-dominated community after the spill. However, knowledge of variability within these communities remains unknown. Herein, we used Illumina high-throughput amplicon sequencing to examine variation throughout a year for the same locations for which the organismal shift was noted. Sediment samples were collected bi-monthly for a year (July 2011-July 2012) from which the meiofaunal community was examined by sequencing the eukaryotic hypervariable V9 region of the 18S rRNA gene. Results showed that the presence of fungal taxa was limited within these communities, suggesting that previously reported acute impacts of the DWHOS on meiofauna were apparently short term. However, these meiofaunal communities show shifts in proportions of metazoan taxa compared to pre-spill samples. Whether this change is due to prolonged impacts of the spill or variation in community composition is unclear. Taxonomic variation within and between sampled locations throughout the study was observed, suggesting potential yearly variation in communities. Continued sampling over a longer timeframe will provide a more complete understanding of seasonality and variation within these communities. Such a baseline is required to assess future anthropogenic impacts.