QUANTITATIVE WESTERN IMMUNOBLOTTING ANALYSIS IN SURVEY OF HUMAN IMMUNODEFICIENCY VIRUS-SEROPOSITIVE PATIENTS
QUANTITATIVE WESTERN IMMUNOBLOTTING ANALYSIS IN SURVEY OF HUMAN IMMUNODEFICIENCY VIRUS-SEROPOSITIVE PATIENTS
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DOI：
10.1128/jcm.27.9.2062-2066.1989
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发表时间：
1989-09-01
影响因子：
9.4
通讯作者：
BAZEX, JA
中科院分区：
文献类型：
--
作者：
SCHIAVINI, DG;PUEL, JML;BAZEX, JA
为了扩大叠氮胸苷抗病毒治疗的适应症并提高其疗效，有必要确定无症状人类免疫缺陷病毒血清阳性患者进展为获得性免疫缺陷综合征的早期预测标准。考虑到这一目标，我们研究了一个队列的血清阳性同性恋男子在法国西南部地区（南比利牛斯山脉）。在分析的所有因素中，第二代酶联免疫吸附试验检测的p24抗体下降被认为是最可靠的。在布隆贝格和施密特工作的基础上，我们开发了一种定量的方法，以蛋白质印迹（免疫印迹）用于人类免疫缺陷病毒感染患者的后续行动。我们的定量蛋白质印迹技术是独特的，因为它使用密度阅读，通过计算机系统，给出一条曲线，允许精确定量每个染色的蛋白质印迹条带。结果表示为积分。该技术证实了p24和p17抗体的降低作为获得性免疫缺陷综合征进展的最早预测标准。
Identifying criteria for the early prediction of progression to acquired immunodeficiency syndrome in asymptomatic human immunodeficiency virus-seropositive patients have become necessary in order to widen the indications for antiviral treatment by azidothymidine and to increase its efficiency. With this aim in mind, we studied a cohort of seropositive homosexual men in the southwest region of France (Midi-Pyrenees). Of all the factors analysed, the decline in p24 antibodies, assayed by second-generation enzyme-linked immunosorbent assay, was found to be the most reliable. On the basis of the work of Blomberg and Schmidt, we developed a quantitative approach to Western blotting (immunoblotting) for use in the follow-up of human immunodeficiency virus-infected patients. Our technique of quantitative Western blotting is unique because it uses a densitometric reading which, through a computerized system, gives a curve allowing the exact quantification of each stained Western blotting band. The results are expressed as integrals. This technique confirms the decrease in p24 and p17 antibodies as the criterion giving the earliest prediction of the progression to acquired immunodeficiency syndrome.