Cryptotomography: Reconstructing 3D Fourier Intensities from Randomly Oriented Single-Shot Diffraction Patterns
Cryptotomography: Reconstructing 3D Fourier Intensities from Randomly Oriented Single-Shot Diffraction Patterns
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DOI：
10.1103/physrevlett.104.225501
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发表时间：
2010-06-02
影响因子：
8.6
通讯作者：
Chapman, H. N.
中科院分区：
文献类型：
--
作者：
Loh, N. D.;Bogan, M. J.;Chapman, H. N.
当明亮、相干、超快的X射线脉冲拦截随机、不可测量方向的单个颗粒时，我们使用DESY的FLASH设施收集的单次2D相干衍射图案重建了单分散扁长纳米颗粒的3D傅里叶强度分布。这第一次实验演示的加密断层扫描扩展的扩展-最大化-压缩框架，以适应不可测量的波动，光子通量和数据丢失，由于饱和或背景散射。这项工作是实现单次衍射成像的单生物分子的重要一步。
We reconstructed the 3D Fourier intensity distribution of monodisperse prolate nanoparticles using single-shot 2D coherent diffraction patterns collected at DESY's FLASH facility when a bright, coherent, ultrafast x-ray pulse intercepted individual particles of random, unmeasured orientations. This first experimental demonstration of cryptotomography extended the expansion-maximization-compression framework to accommodate unmeasured fluctuations in photon fluence and loss of data due to saturation or background scatter. This work is an important step towards realizing single-shot diffraction imaging of single biomolecules.