A Validated Stability-Indicating HPTLC Assay for Determination of 10-Hydroxy-2-Decenoic Acid Content in Royal Jelly Products Using Robust Regression Methods
A Validated Stability-Indicating HPTLC Assay for Determination of 10-Hydroxy-2-Decenoic Acid Content in Royal Jelly Products Using Robust Regression Methods
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DOI：
10.1093/chromsci/bmaa016
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发表时间：
2020-07-01
影响因子：
1.3
通讯作者：
El-Banna, Alaa A.
中科院分区：
文献类型：
--
作者：
Korany, Mohamed A.;Moneeb, Marwa S.;El-Banna, Alaa A.
建立了一种简便、稳定的高效薄层色谱法，用于定量埃及部分蜂王浆产品中10-羟基-2-十烯酸（10-HDA）的含量。所用溶剂体系为氯仿：乙酸（10:1,v/v），在210 nm处进行密度测定。对数据进行了一阶和二阶导数处理。本研究比较了三种统计回归方法：参数、非参数和加权回归（WR）方法。根据国际协调会议的准则对所制定的方法进行了验证。为了验证所建立的分析方法的稳定性指示能力，对蜂王浆标准品进行了强制降解研究，包括水解、氧化、光解和干热的影响。结果表明，对色谱响应数据进行导数处理可以提高色谱信号的定量和灵敏度。发现响应数据的加权回归比使用参数和非参数回归模型更有利。在蜂王浆产品中10-HDA分析的准确性和精密度上有很大的提高。WR的回收率为99.92 +/- 0.16，非参数和参数回归的回收率分别为99.56 +/- 0.25和98.63 +/- 0.65。
A( )new, simple, stability-indicating high-performance thin-layer chromatography method was developed for the quantification of 10-hydroxy-2-decenoic acid (10-HDA) in some royal jelly products marketed in Egypt. The used solvent system was chloroform:acetic acid (10:1, v/v) and the bands were measured densitometrically at 210 nm. First- and second-derivative treatments of the data were performed. The present study shows a comparison between three statistical regression methods for handling data: parametric, nonparametric and weighted regression (WR) methods. The developed methods were validated as per International Conference on Harmonization guidelines. To validate the stability-indicating power of the developed analytical method, the royal jelly standard was subjected to forced degradation studies including the effect of hydrolysis, oxidation, photolysis and dry heat. It was found that derivative treatment of the chromatographic response data gives improved quantitation and sensitivity of the chromatographic signals. Weighted regression of the response data is found to be advantageous over the use of both parametric and nonparametric regression models. This was shown by a great enhancement in the accuracy and precision in the analysis of 10-HDA in royal jelly products. The % recovery in case of WR was 99.92 +/- 0.16, while % recovery in case of nonparametric and parametric regressions were 99.56 +/- 0.25 and 98.63 +/- 0.65, respectively.