CmMYB19 Over-Expression Improves Aphid Tolerance in Chrysanthemum by Promoting Lignin Synthesis.
CmMYB19 Over-Expression Improves Aphid Tolerance in Chrysanthemum by Promoting Lignin Synthesis.
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CmMYB19过表达通过促进木质素合成提高菊花的蚜虫耐受性
DOI：
10.3390/ijms18030619
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发表时间：
2017-03-12
影响因子：
5.6
通讯作者：
Chen S
中科院分区：
文献类型：
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作者：
Wang Y;Sheng L;Zhang H;Du X;An C;Xia X;Chen F;Jiang J;Chen S
从菊花中分离得到禽成髓细胞病病毒L癌基因同源转录因子MYB(v-MYB)基因CmMYB19。它编码一个200个氨基酸的蛋白质，属于R2R3-MYB亚家族。CmMYB19在酵母中没有转录激活，而在洋葱表皮细胞中进行的瞬时表达实验表明，CmMYB19产物定位于细胞核。CmMYB19的转录受棉铃虫侵染诱导，在叶和茎中的丰度高于根。CmMYB19基因的过度表达限制了麦长管蚜的增殖。与木质素合成相关的主要基因的转录丰度比较表明，CmPAL1(苯丙氨酸解氨酶1)、CmC4H(肉桂酸4羟基酶)、Cm4CL1(4-羟基肉桂酰辅酶A连接酶1)、CmHCT(羟基肉桂酰辅酶A/奎宁酸羟基肉桂酰基转移酶)、CmC3H1(香豆酸3羟基酶1)、CmCCoAOMT1(咖啡酰辅酶A O-甲基转移酶1)和CmCCR1(肉桂酰辅酶A还原酶1)都表达上调，这与CmMYB19表达植物中木质素含量的增加一致。总体而言，CmMYB19的过度表达通过促进木质素的积累限制了棉铃虫在寄主上的繁殖。
The gene encoding the MYB (v-myb avian myeloblastosis vira l oncogene homolog) transcription factor CmMYB19 was isolated from chrysanthemum. It encodes a 200 amino acid protein and belongs to the R2R3-MYB subfamily. CmMYB19 was not transcriptionally activated in yeast, while a transient expression experiment conducted in onion epidermal cells suggested that the CmMYB19 product localized to the nucleus. CmMYB19 transcription was induced by aphid (Macrosiphoniella sanborni) infestation, and the abundance of transcript was higher in the leaf and stem than in the root. The over-expression of CmMYB19 restricted the multiplication of the aphids. A comparison of transcript abundance of the major genes involved in lignin synthesis showed that CmPAL1 (phenylalanine ammonia lyase 1), CmC4H (cinnamate4 hydroxylase), Cm4CL1 (4-hydroxy cinnamoyl CoA ligase 1), CmHCT (hydroxycinnamoyl CoA-shikimate/quinate hydroxycinnamoyl transferase), CmC3H1 (coumarate3 hydroxylase1), CmCCoAOMT1 (caffeoyl CoA O-methyltransferase 1) and CmCCR1 (cinnamyl CoA reductase1) were all upregulated, in agreement with an increase in lignin content in CmMYB19 over-expressing plants. Collectively, the over-expression of CmMYB19 restricted the multiplication of the aphids on the host, mediated by an enhanced accumulation of lignin.