Alterations in neuronal development in the substantia nigra pars compacta following in utero ethanol exposure: immunohistochemical and Golgi studies.
Alterations in neuronal development in the substantia nigra pars compacta following in utero ethanol exposure: immunohistochemical and Golgi studies.
复制标题
子宫内乙醇暴露后黑质致密部神经元发育的改变：免疫组织化学和高尔基体研究。
DOI：
10.1016/0306-4522(93)90159-d
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发表时间：
1993
期刊：
影响因子：
3.3
通讯作者：
Phillips,DE
中科院分区：
文献类型：
--
作者：
Shetty,AK;Burrows,RC;Phillips,DE
研究了妊娠期乙醇暴露对大鼠黑质致密部多巴胺能神经元发育的影响。怀孕大鼠在整个妊娠期间被喂食含乙醇的液体饮食（6.7% v/v）或成对喂食等热量饮食。采用酪氨酸羟化酶免疫组织化学和高尔基浸渍法，在出生后第15天对乙醇暴露和配对喂养的对照组小鼠的神经元形态进行了评估。与对照组相比，暴露于乙醇的后代神经元发育发生了以下变化：(i)酪氨酸羟酶阳性的细胞体更小，排列更紧密；（ii）每个细胞的二级、三级和四级树突数量和总树突节段减少；（iii）相对于离体细胞距离的树突分支模式发生改变；（iv）酪氨酸羟化酶免疫染色和高尔基染色标本中均出现了细胞体轮廓不规则和树突球形增大的畸形神经元。本研究结果提示，妊娠期乙醇暴露可导致黑质致密部神经元发育迟缓，尤其是树突生长和分支发育迟缓，并引起部分神经元的病理改变。树突发育不全可能导致神经元回路发育改变，进而导致运动功能异常。
The effects of gestational ethanol exposure on the development of dopaminergic neurons of substantia nigra pars compacta were investigated in the rat. Pregnant rats were either fed an ethanol-containing liquid diet (6.7% v/v) or pair-fed an isocaloric diet throughout gestation. The morphology of neurons in both ethanol-exposed and pair-fed control offspring was assessed on postnatal day 15 by using tyrosine hydroxylase immunohistochemistry and Golgi impregnation methods. Alterations in the development of neurons were indicated in ethanol-exposed offspring compared with control offspring by the following:(i)tyrosine hydroxylase-positive cell bodies were smaller and appeared more closely packed;(ii)the numbers of second-, third- and fourth-order dendrites and total dendritic segments per cell were reduced;(iii)the dendritic branching pattern relative to distance from the soma was altered; and(iv)some dysmorphic neurons with irregular cell body contours and spheroidal enlargements in the dendrites were encountered in both tyrosine hydroxylase-immunostained and Golgi-stained specimens.The results of the present study suggest that gestational ethanol exposure causes retardation in the development of substantia nigra pars compacta neurons, especially in their dendritic growth and branching, and also causes pathological changes in some neurons. The underdevelopment of dendrites could result in altered development of neuronal circuitry which, in turn, could result in abnormal motor function.