Anti-inflammatory and anti-arthritic activity of a methanol extract from Vitellaria paradoxa stem bark.
Anti-inflammatory and anti-arthritic activity of a methanol extract from Vitellaria paradoxa stem bark.
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DOI：
10.4103/0974-8490.159569
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发表时间：
2014-10
影响因子：
0.7
通讯作者：
Oben EK
中科院分区：
文献类型：
--
作者：
Foyet HS;Tsala DE;Zogo Essono Bodo JC;Carine AN;Heroyne LT;Oben EK
牡丹是喀麦隆的一种传统药用植物。对这种植物的几项研究都集中在其果实的美容特征上。本研究主要对该植物树皮提取物的抗炎作用进行研究。目的：观察异花弧菌树皮甲醇提取物(VPME)对大鼠炎性反应的影响。采用Wistar大鼠急性炎症和慢性炎症(28d)模型，观察VPME的抗炎作用。对痛觉过敏和运动活动的影响也被量化。测定淋巴器官的相对重量和部分血液学指标。在角叉菜胶诱导的炎症反应中，VPME(75 mg/kg)在1h后显著抑制(66.67%)，在完全弗氏佐剂诱导的类风湿关节炎中，VPME在治疗2d后对对照组的炎症反应有显著的保护作用，炎症反应的抑制率为8.12%，对照组的炎症抑制率为25.00%。提取物(75 mg/kg和150 mg/kg)能显著降低小鼠关节炎的评分，在实验第19天达到最大值。大鼠在热板上的反应时间明显增加，动物在野外的探索活动也明显增加。这种提取物显著地防止体重、血红蛋白和红细胞的损失，以及脾肥大。对皮肤破坏和软骨侵蚀有明显的保护作用。液-质联用分析表明，提取物中含有儿茶素。这些结果表明，反常弧菌可能有助于减轻炎症反应。
Vitellaria paradoxa is a traditional medicinal plant of Cameroon. Several studies on this plant have focused on the cosmetic profile of its fruits. The present study focuses on the anti-inflammatory potency of stem barks extract of this plant. The objective was to evaluate the effect of methanolic extract of V. paradoxa (VPME) stem barks on inflammatory response in rats. Anti-inflammatory effects of VPME were evaluated in acute and chronic (28 days) inflammation induced in Wistar albino rats. The effects on hyperalgesia and locomotors activity were also quantified. The relative weight of lymphoid organs was obtained as well as some hematological parameters. In the carrageenan-induced inflammation, VPME (75 mg/kg) exhibited a significant (66.67%) inhibition after 1 h. On the complete Freund's adjuvant-induced rheumatoid arthritis, VPME showed a significant protective effect with 8.12% inflammation against 25.00% for the control group after 2 days of the treatment. The extract (75 and 150 mg/kg) significantly reduced the score of arthritis with a maximum obtained on day 19th of the experimentation. There was a significant increase in the reaction time of rats on the hot plate as well as the exploratory activities of the animals in the open field. This extract significantly prevented weight, hemoglobin and red blood cells losses, and spleen hypertrophy. A protective action against skin destruction and cartilage erosion was evident. Liquid chromatography-mass spectrometry analysis of the extract revealed the presence of catechins. These findings suggested that V. paradoxa may contribute to the reduction of the inflammatory response.