ACCESSIBILITY OF IODOPSIN CHROMOPHORE
ACCESSIBILITY OF IODOPSIN CHROMOPHORE
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DOI：
10.1016/0304-4165(75)90337-2
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发表时间：
1975-01-01
期刊：
影响因子：
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通讯作者：
YOSHIZAWA, T
中科院分区：
文献类型：
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作者：
MATSUMOTO, H;TOKUNAGA, F;YOSHIZAWA, T
碘视蛋白可以通过添加9-顺式视黄酸来取代其发色团（11-顺式视黄酸），导致异碘视蛋白的形成。NaBH 4在黑暗中漂白碘视蛋白。在相对低浓度的毛地黄皂苷中，暗视蛋白（鸡视紫红质的蛋白质部分）从碘视蛋白中的异辛中除去11-顺式视黄。这些事实表明，碘视蛋白分子中的发色团与视蛋白的连接（推测为希夫碱连接）对这些试剂是可接近的，这与视紫红质中的情况不同。
Iodopsin can replace its chromophore (11-cisretinal) by added 9-cisretinal, resulting in the formation of isoiodopsin. NaBH4bleaches iodopsin in the dark. In a relatively low concentration of digitonin, the scotopsin (the protein moiety of chicken rhodopsin) removes 11-cisretinal from isopsin in the iodopsin These facts suggests that the linkage of the chromophore to opsin in the iodopsin molecule (presumably a Schiff-base linkage) is accessible to these reagents, which is different from the situation in rhodopsin.