A green carbon dots-based fluorescent sensor for selective and visual detection of nitrite triggered by the nitrite-thiol reaction
A green carbon dots-based fluorescent sensor for selective and visual detection of nitrite triggered by the nitrite-thiol reaction
复制标题
基于绿色碳点的荧光传感器，用于选择性和视觉检测亚硝酸盐-硫醇反应引发的亚硝酸盐
DOI：
10.1039/d0nj01025a
复制
发表时间：
2020
影响因子：
3.3
通讯作者：
Bai Yanhong
中科院分区：
文献类型：
--
作者：
Yue Xiaoyue;Zhou Zijun;Wu Yongmei;Jie Mingsha;Li Yan;Guo Haobin;Bai Yanhong
Nitrite misuse seriously threatens food safety, therefore, it is of significance to develop a facile, sensitive and selective methodology for trace detection of nitrite in food samples for ensuring people's health and safety. Herein, a novel fluorescent sensor was successfully designed for selective, sensitive and visual detection of nitrite. This sensing mechanism involves the inner-filter effect between synthetic green carbon dots and the S-nitrosothiol compound. Green carbon dots were synthesized using a one-step hydrothermal method and used as fluorescent probes, and S-nitrosothiol was generated through the π-conjugation triggered nitrite–thiol reaction. Selective and sensitive nitrite detection was realized using a fluorescence spectrophotometer in the detection range of 0.4–20 μg mL−1. The detection limit of the developed method was calculated to be 0.23 μg mL−1 with satisfactory recoveries ranging from 86.61% to 103.22%. Upon illumination with the help of a portable ultraviolet lamp, the brightness and color of the green fluorescent probe changed with the addition of nitrite at different concentrations. Visual changes were directly observed with the naked eye and used for the qualitative and semiquantitative analysis of nitrite contents. The developed fluorescent sensor offers an effective, simple, rapid and low cost analytical method for sensitive and visual nitrite detection in meat samples to monitor and reduce the risk of nitrite overuse in food processing.
