A unique influenza A (H5N1) virus causing a focal poultry outbreak in 2007 in Manipur, India.
A unique influenza A (H5N1) virus causing a focal poultry outbreak in 2007 in Manipur, India.
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DOI：
10.1186/1743-422x-6-26
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发表时间：
2009-02-24
期刊：
影响因子：
4.8
通讯作者：
Kode SS
中科院分区：
文献类型：
--
作者：
Mishra AC;Cherian SS;Chakrabarti AK;Pawar SD;Jadhav SM;Pal B;Raut S;Koratkar S;Kode SS
2007年，印度东北部的曼尼普尔邦报告了一起家禽H5 N1疫源性疫情。本研究的目的是对曼尼普尔分离株进行基因鉴定，以了解与其他H5 N1分离株的关系，并追踪该病毒传入该国的可能来源。完整基因组的特征显示该病毒属于进化枝2.2。该病毒与进化枝2.2的3个EMA亚系的病毒明显不同，但与来自俄罗斯、中国和蒙古的野生迁徙水禽分离物相关。HA基因具有切割位点GERRRRKR，早在2005年在蒙古的大天鹅分离物中报道。PB 1-F2蛋白中29位的终止密码子可能对复制效率有影响。2005- 2007年从不同地理区域分离的病毒株中发现的多态性表明，H5 N1病毒可能通过候鸟传播。考虑到早期印度分离株的所有8个基因都属于EMA 3亚系，而且次大陆邻国没有报告类似的毒株，看来该病毒可能是独立传入的。
A focal H5N1 outbreak in poultry was reported from Manipur, a north-eastern state, of India, in 2007. The aim of this study was to genetically characterize the Manipur isolate to understand the relationship with other H5N1 isolates and to trace the possible source of introduction of the virus into the country. Characterization of the complete genome revealed that the virus belonged to clade 2.2. It was distinctly different from viruses of the three EMA sublineages of clade 2.2 but related to isolates from wild migratory waterfowl from Russia, China and Mongolia. The HA gene, had the cleavage site GERRRRKR, earlier reported in whooper swan isolates from Mongolia in 2005. A stop codon at position 29 in the PB1-F2 protein could have implications on the replication efficiency. The acquisition of polymorphisms as seen in recent isolates of 2005–07 from distinct geographical regions suggests the possibility of transportation of H5N1 viruses through migratory birds. Considering that all eight genes of the earlier Indian isolates belonged to the EMA3 sublineage and similar strains have not been reported from neighbouring countries of the subcontinent, it appears that the virus may have been introduced independently.