Upregulation of CENPM facilitates lung adenocarcinoma progression via PI3K/AKT/mTOR signaling pathway.
Upregulation of CENPM facilitates lung adenocarcinoma progression via PI3K/AKT/mTOR signaling pathway.
复制标题
DOI：
10.3724/abbs.2021013
复制
发表时间：
2022-01-25
影响因子：
3.7
通讯作者：
Deng Z
中科院分区：
文献类型：
--
作者：
Liu C;Wang Y;Dao Y;Wang S;Hou F;Yang Z;Liu P;Lv J;Lv L;Li G;Zhou Y;Deng Z
Centromere protein M (CENPM) is essential for chromosome separation during mitosis. However, its roles in lung adenocarcinoma (LUAD) progression and metastasis remain unknown. In this study, we aimed to explore the effects of CENPM on LUAD progression as well as the underlying mechanisms. We analyzed the expression of CENPM and its correlation with clinicopathological characteristics using GEO LUAD chip datasets and TCGA dataset. We further investigated the impact of CENPM on LUAD in vitro and in vivo. In silico analysis and qRT-PCR revealed that CENPM is upregulated in LUAD compared with that in normal lung tissues. Via gain/loss-of-function assays, we further found that CENPM promotes the LUAD cell cycle, cell proliferation, migration and invasion, and inhibits cell apoptosis in vitro. The in vivo study showed that loss of CENPM inhibits the growth of A549 xenografts. Furthermore, we found that CENPM can promote the phosphorylation of mTOR rather than directly affect the mTOR content. Inhibition of mTOR activity abrogates the promoting effects of CENPM on cell cycle progression, cell proliferation, migration and invasion. Taken together, these results show that CENPM plays an important role in the growth and metastasis of LUAD and may be a promising therapeutic target in LUAD.
登录
查看更多内容
影响因子：
20.4
作者：
Zhang, Yanting;Luo, Ganfeng;Hao, Yuantao
通讯作者：
Hao, Yuantao
影响因子：
2.1
作者：
Aguilar-Serra, Javier;Gimeno-Ballester, Vicente;Cortijo, Julio
通讯作者：
Cortijo, Julio
影响因子：
5.8
作者：
Wu, Zeng-hong;Yang, Dong-liang
通讯作者：
Yang, Dong-liang
影响因子：
7.2
作者：
Zhao, Liqun;Zhu, Lei;Sun, Shi-Yong
通讯作者：
Sun, Shi-Yong
影响因子：
4.2
作者：
Zheng, Chenlei;Zhang, Tan;Chen, Bicheng
通讯作者：
Chen, Bicheng