Natural honey prevents ischaemia-reperfusion-induced gastric mucosal lesions and increased vascular permeability in rats
Natural honey prevents ischaemia-reperfusion-induced gastric mucosal lesions and increased vascular permeability in rats
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DOI：
10.1097/00042737-199711000-00014
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发表时间：
1997-11-01
影响因子：
2.1
通讯作者：
AlTuwaijiri, AS
中科院分区：
文献类型：
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作者：
Ali, ATMM;AlSwayeh, OA;AlTuwaijiri, AS
目的：有人认为天然蜂蜜中可能含有硫糖铝样物质。最近的研究表明，硫糖铝对大鼠胃缺血再灌注损伤具有保护作用。因此，研究蜂蜜对大鼠胃缺血再灌注损伤、肠腔内出血、血管通透性和胃内非蛋白巯基(NP-SH)的影响。方法：用100 mM盐酸造成大鼠胃缺血30min，再灌流60min。大体观察管腔内出血情况，倒置显微镜下对胃部病变进行分级。血管通透性通过分光光度法测量胃中渗出的伊文思蓝染料来定量。用分光光度法测定血浆NP-SH水平。采用鲁米诺依赖化学发光法检测蜂蜜的体外抗氧化作用。结果：与对照组比较，再灌注期大鼠胃粘膜损伤明显增多，肠腔内出血明显增多，伊文思蓝渗漏增多，NP-SH耗竭。缺血再灌流前30min给予蜂蜜(0.078~0.625 g/kg，口服)或二甲基亚砜(0.02~0.08g/kg，腹腔注射)，可剂量依赖地减少胃损伤和管腔出血，降低血管通透性。此外，蜂蜜还能逆转缺血再灌注引起的NP-SH水平的降低，并抑制无细胞黄嘌呤-黄嘌呤氧化酶系统诱导的鲁米诺依赖的化学发光。结论：蜂蜜的胃保护作用可能是其抗氧化作用的结果。有人认为，蜂蜜的这一特性可能是由于存在一种类似硫糖铝的物质。
Objective: It has been proposed that natural honey may contain a `sucralfate-like' substance. Recent studies have shown that sucralfate affords protection against ischaemia-reperfusion-induced injuries in the rat stomach. Therefore, the effect of honey was studied on ischaemia-reperfusion-induced gastric lesions, intraluminal bleeding, vascular permeability and non-protein sulphhydryls (NP-SH) in the rat stomach.Methods: Rats were subjected to 30 min of gastric ischaemia in the presence of 100 mM HCl and reperfusion period of 60 min. Intraluminal bleeding was assessed macroscopically and the gastric lesions were graded microscopically under an inverted microscope. Vascular permeability was quantified by measuring spectrophotometrically the extravasated Evans blue dye in the stomach. NP-SH levels were measured spectrophotometrically. A luminol-dependent chemiluminescence method was used to assess antioxidant effects of honey in vitro.Results: There were significantly more gastric lesions, more severe intraluminal bleeding, more leakage of Evans blue and depletion of NP-SH during the reperfusion period as compared to controls. Pre-treatment with honey (0.078-0.625 g/kg, orally) or dimethyl sulphoxide (0.02-0.08 g/kg, intraperitoneally) 30 min before the ischaemia-reperfusion dose-dependently reduced the gastric lesions and intraluminal bleeding and decreased the vascular permeability. Furthermore, honey reversed the ischaemia-reperfusion-induced depletion of NP-SH levels and inhibited the luminol-dependent chemiluminescence induced in a cell-free xanthine-xanthine oxidase system.Conclusion: These results suggest that gastric protection by honey may be a result of its antioxidant effect. It is suggested that this property of honey may be due to the presence of a `sucralfate-like' substance.