Diversity in populations of free-living Symbiodinium from a Caribbean and Pacific reef
Diversity in populations of free-living Symbiodinium from a Caribbean and Pacific reef
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DOI：
10.4319/lo.2008.53.5.1853
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发表时间：
2008-09-01
影响因子：
4.5
通讯作者：
Gates, Ruth D.
中科院分区：
文献类型：
--
作者：
Manning, Mackenzie M.;Gates, Ruth D.
自由生活的腰鞭毛虫属于共生属共生藻的存在和多样性进行了探讨，在上述珊瑚礁在凯恩奥赫湾，夏威夷，和波多黎各莫雷洛斯，金塔纳罗奥，墨西哥的海水样品。两个遗传标记被用来评估共生藻的多样性在水柱：内部转录间隔区2区的核核糖体阵列（ITS 2），和高变区的结构域V的大亚基（23 S）的叶绿体核糖体阵列（cp 23 S-HVR）。克隆的基因片段的测序显示，分支B，C，D，和H共生藻在海水样品中是可检测的。除了先前描述的类型B1、C3、C15、C21和D1之外，还检索到属于进化枝B和C的新的共生藻序列。大部分的共生藻序列从凯恩奥赫湾属于C支和那些从波多黎各莫雷洛斯的分支B，模式，反映了优势类型的共生藻内共生与石珊瑚在这两个领域。这项研究是第一次直接评估位于加勒比和太平洋的珊瑚礁附近的沃茨中的共生藻多样性，并证实了共生藻在生态系统的这一部分中的存在。这些数据为今后的研究提供了背景，研究共生甲藻在水柱中的可用性的空间和时间模式，这项工作最终将促进对共生甲藻及其环境敏感的底栖宿主之间的相互作用的更好理解。
The presence and diversity of free-living dinoflagellates belonging to the endosymbiotic genus Symbiodinium were explored in seawater samples collected above coral reefs in Kane'ohe Bay, O'ahu, Hawai'i, and Puerto Morelos, Quintana Roo, Mexico. Two genetic markers were used to assess Symbiodinium diversity in the water column: the internal transcribed spacer 2 region of the nuclear ribosomal array (ITS2), and a hypervariable region in domain V of the large subunit (23S) of the chloroplast ribosomal array (cp23S-HVR). Sequencing of cloned gene fragments reveals that clades B, C, D, and H Symbiodinium are detectable in the seawater samples. In addition to the previously described types B1, C3, C15, C21, and D1, novel Symbiodinium sequences belonging to clades B and C were also retrieved. The majority of Symbiodinium sequences recovered from Kane'ohe Bay belonged to clade C and those from Puerto Morelos to clade B, a pattern that reflects the dominant types of Symbiodinium found in endosymbiosis with scleractinian corals in these two areas. This study represents the first direct assessment of Symbiodinium diversity in waters adjacent to coral reefs located in the Caribbean and the Pacific and confirms the presence of Symbiodinium in this compartment of the ecosystem. These data provide context for future studies examining spatial and temporal patterns in the availability of Symbiodinium in the water column, work that will ultimately promote a greater understanding of the interactions between symbiotic dinoflagellates and their environmentally sensitive benthic hosts.