Observations on the nature of a genetic cellular resistance to avian tumor viruses.
Observations on the nature of a genetic cellular resistance to avian tumor viruses.
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对禽肿瘤病毒的遗传细胞抗性性质的观察。
DOI：
10.1093/jnci/41.1.145
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发表时间：
1968
期刊：
影响因子：
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通讯作者：
Lyman B. Crittenden
中科院分区：
文献类型：
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作者：
Lyman B. Crittenden
选择劳斯肉瘤病毒Bryan高滴度毒株（RSV (rav1)）的A亚群假型和对A亚群（C/A和C/AB）耐药的鸡胚成纤维细胞进行研究。用游离病毒感染耐药细胞的尝试表明，即使不是绝对的，耐药也是非常有效的。抗性细胞从培养基中清除病毒的效率与敏感细胞一样高，这表明遗传细胞抗性不影响吸附。然而，C/A和C/AB细胞在与辐照致死性RSV （rv -1）产生易感细胞接触生长后，均能合成RSV （rv -1）。因此，基因控制的阻断显然发生在渗透或剥膜的水平，但不影响细胞合成被排除的病毒的能力，前提是病毒基因组已经进入。在与辐照产生病毒的细胞共培养过程中，病毒基因组渗透到抗性细胞中的机制尚不清楚。讨论了可能的机制。
The subgroup A pseudotype of the Bryan high-titer strain of Rous sarcoma virus [RSV (RAV-1)] and chick embryo fibroblasts resistant to subgroup A (C/A and C/AB) were chosen for study. Attempts to infect resistant cells with free virus showed that resistance was very efficient, if not absolute. Resistant cells removed virus from the medium as efficiently as susceptible cells, which indicated that genetic cellular resistance did not affect adsorption. However, both C/A and C/AB cells synthesized RSV (RAV-1) after growth in contact with lethally irradiated RSV (RAV-1)-producing susceptible cells. Therefore, the genetically controlled block apparently occurred at the level of penetration or uncoating, but did not affect the cell's ability to synthesize the excluded virus, provided the viral genome had entered. The mechanism of penetration of the viral genome into resistant cells during cocultivation with irradiated virus-producing cells is not clear. Possible mechanisms are discussed.